猪胰岛细胞分离纯化研究进展  

PROGRESS IN ISOLATION AND PURIFICATION OF PORCINE ISLETS

作　　者：朱海涛[1] 于良[1] 王博[1] 

机构地区：[1]西安交通大学医学院第一附属医院肝胆外科,西安710061

出　　处：《中国修复重建外科杂志》2012年第8期1012-1016,共5页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金资助项目(30700772)~~

摘　　要：目的综述猪胰岛细胞分离纯化的常用方法及最新研究进展。方法查阅近年来国内外关于猪胰岛细胞分离纯化的相关文献,进行总结分析。结果分离纯化的效果主要取决于供体的前期筛选、高质量胰腺的获取与有效保存,以及分离纯化方法的选择与改进。结论猪胰岛分离纯化操作的优化与标准化的建立,极大促进了异种胰岛移植研究的发展,有望解决目前移植供体短缺的问题。Objective To review the common methods of isolation and purification of porcine islets and research progress. Methods Domestic and abroad literature concerning the isolation and purification of porcine islets was reviewed and analyzed thoroughly. Results The efficacy of the isolation and purification depends on the selection of donor, the procurement and cryopreservation of high-quality donor pancreas, and the selection and improvement of the operation. Conclusion The shortage of transplanted islets could be resolved by the establishment of standardized and ootimal orocess, which may also oromote the develooment of oorcine islet xenograft.

关 键 词：胰岛细胞 分离 纯化 异种移植 猪 

分 类 号：R587.1[医药卫生—内分泌]