共生菌普拉梭菌对大鼠实验性结肠炎的保护作用及机制研究  

Protective effects and mechanisms of Faecalibacterium prausnitzii in experimental colitis in rats

作　　者：张明明[1] 杨晓彤[1] 邱新运[1] 洪娜[1] 于成功[1] 

机构地区：[1]南京大学医学院附属鼓楼医院消化科,210008

出　　处：《中华消化杂志》2012年第8期549-554,共6页Chinese Journal of Digestion

基　　金：国家自然科学基金面上项目（81170359）

摘　　要：目的研究普拉梭菌（F户）及其产物对2，4，6三硝基苯磺酸（TNBS）诱导的大鼠实验性结肠炎的保护作用和机制。方法40只SD大鼠分为健康对照组、结肠炎模型组、Fp上清液组、F声活菌组、双歧杆菌组，后4组用TNBS灌肠造模并于造模前5d和造模后ld分别用PBS、F户上清液、Fp活菌、双歧杆菌活菌灌胃。造模48h后，处死大鼠，取结肠行病理学检查；用气相色谱法检测结肠粪便短链脂肪酸（SCFA）含量；ELISA法检测血浆IL-10、IL-12、IL-17和IL-23水平；免疫组织化学检测肠黏膜IL-17的表达。组间比较采用单因素方差分析。结果与健康对照组大鼠相比，结肠炎模型组大鼠体质量下降、组织学评分增加[（193．57±14）g比（170．25±19．18）g，（1．00±0．99）分比（3．34±0．38）分，t值分别-2．83和7．55，P值均〈0．053。各治疗组中F户上清液组效果最为明显，体质量和组织学评分显著优于结肠炎模型组[（187．00±14．67）g，（2．50±0．44）分，t值分别-2．1和2．9，P值均〈O．053。与健康对照组相EB，结肠炎模型组大鼠血浆和结肠组织中的IL，17表达水平均上调[（16．61士2．45）pg／ml比（20．47±1．45）pg／ml，（O．83±0．98）分比（5．14±0．90）分，t=2．88、7．84，P值均〈0．05）]。与结肠炎模型组相比，Fp上清液组的血浆和结肠组织IL-17表达则明显下调[分别为（17．54±1．51）pg／ml和（2．86±0．69）分，t=2．1和2．9，P值均〈0．053。结论Fp菌可以调节免疫反应，抑制大鼠结肠黏膜炎性反应，其作用可能和抑制IL-17表达有关。Objective To investigate the protective effects and mechanism of Faecalibacterium prausnitzii （Fp） and its products in 2,4,6-trinitrobenzene sulfonic acid （TNBS） induced colitis rats. Methods A total of 40 Sprague-Dawley rats were divided into healthy control group, colitis model group, Fp supernatant group, Fp bacteria group and Bifidobacterium longum （B. longum） group. The rats of the later four groups were enemaed with TNBS to establish the model. At five days before and one day after modeling, the rats were gavaged with phosphate buffer saline （PBS）, the supernatant of Fp, live Fp bacteria and live B. longum respectively. Rats were executed at 48 hour after modeling. The colon tissues were taken for pathology examination. The content of short-chain fatty acids （SCFA） in fecal was tested by gas chromatography. The plasma level of interleukin-10 （IL- l0）, interleukin-12 （[L-12）, interleukin-17 （IL-17） and interleukin-23 （IL-23） were detected by enzyme-linked immunosorbnent assay （ELISA） and the expression of IL-17 in intestinal mucosal tissues was measured by immunohistochemistry. The data were analyzed by one way ANOVA. Results Compared with the healthy control group, the rats of colitis group suffered serious weight loss and their intestinal pathology score increased I-（193.57±14） g vs （170.25±19.18） g, （1.00±0.99） vs （3.34±0.38） ,t= 2.83 and 7.55, all P value〈0. 051. The Fp supernatant group showed protective effects in terms of weight and intestinal pathology score （187.00±14, 67） g, （2.50±0.44）, t=2.1 and 2.9, all P〈0. 051. Compared with healthy control group, the plasma and colon tissue ILd7 concentration of colitis model group increased （16.61 pg/ml±2.45 pg/ml vs 20.47 pg/ml±l. 45 pg/ml, 0.83±0.98 vs 5.14±0.90） （all P〈0.05）. Compared with the colitis model group, the plasma and colon tissue IL-17 concentration of Fp supernatant group decreased （17.54 pg/ml ± 1. 51 pg/ml and 2.86±0.69）. Conclusion Fp can regulat

关 键 词：共生 结肠炎 微生物学 三硝基苯磺酸 白细胞介素17 

分 类 号：R285.5[医药卫生—中药学]