2009-2010年黑龙江中俄边境口岸鼠类动物中汉坦病毒分子流行情况调查及分析  

Epidemiologic investigation and analysis on Hantavirus carried by rodents at Heilongjiang frontier ports between Sino-Russian border in 2009～2010

作　　者：侯咏 李大鹏[2] 刘宇鹏[2] 鞠文东 程成 高丹凝 富英群 赵亚双[2] 

机构地区：[1]黑龙江出入境检验检疫局卫检处,黑龙江哈尔滨150090 [2]哈尔滨医科大学

出　　处：《中国国境卫生检疫杂志》2012年第3期186-189,共4页Chinese Journal of Frontier Health and Quarantine

基　　金：国家质检总局科研基金项目(2010IK218)

摘　　要：目的研究黑龙江省中俄边境6个口岸鼠类动物的种类和携带汉坦病毒情况及病毒基因分型,为口岸防控肾综合症出血热提供科学依据。方法采用夹夜法和鼠笼法捕获鼠类,荧光定量PCR(TaqMan探针法)对鼠肺标本进行检测。结果此次调查共捕获鼠类动物295只,经鉴定隶属3科8属10种,其中黑线姬鼠为优势种群,占42.37%。检测出汉坦病毒核酸阳性鼠肺标本22份,鼠总带毒率为7.46%,其中,汉滩型15份,汉城型共7份。在调查的6个口岸中逊克、抚远、黑河、东宁和绥芬河5个口岸发现了汉坦病毒核酸阳性标本。结论本次共调查黑龙江省中俄边境6个口岸,其中有5个口岸发现了汉坦病毒核酸阳性标本,北部边境逊克、抚远和黑河3个口岸均发现了汉坦病毒核酸阳性标本,东部边境东宁、绥芬河、牡丹江3个口岸中东宁和绥芬河发现了汉坦病毒核酸阳性标本,黑龙江省口岸肾综合症出血热防控形势依然严峻。Objective To investigate the rodent species and Hantavirus infections at Heilongjiang frontier ports between Sino-Russia border, and to provide scientific evidence for hemorrhagic fever with renal syndrome prevention. Methods Night trapping method and mouse cages were used to capture rodents. The rodent lung tissues were detected by real-time fluorescence quantitative PCR. Results Two hundred and ninety five captured rodents were identified as 3 families 8 genera 10 species. Apodmus agrarius was the dominant specie. A total of 22 samples carried Hantavirus with the infection rate of 7.46%. In which, 15 samples belonged to HNTV and 7 samples belonged to SEOV. Hantaviruses were carried by rodents in Xunke, Fuyuan, Heibe, Dongning, Suifenhe of Heilongjiang province. Conclusion Hantaviruses were carried by rodents at five ports between Sido-Russia border in this study. The situation of hemorrhagic fever with renal syndrome prevention was still grim.

关 键 词：汉坦病毒 鼠类动物 荧光定量PCR 基因分型 

分 类 号：R512.8[医药卫生—内科学] R184.38[医药卫生—临床医学]