动物产品及饲料中牛源和羊源性成分三重荧光PCR检测方法的建立  

Establishment of multiplex fluorescent PCR assay for the detection of bovine,goat and sheep-derived materials in animal products and feeding stuffs

作　　者：赵冉 蔡振鸿 陈永锋 

机构地区：[1]厦门市农产品质量安全检验测试中心,福建厦门361012 [2]新奥(厦门)农牧发展有限公司,福建厦门361000

出　　处：《畜牧与兽医》2012年第7期18-22,共5页Animal Husbandry & Veterinary Medicine

摘　　要：为鉴定和区分饲料及动物产品中牛、山羊、绵羊源性成分,根据线粒体DNA(mitochondrial DNA,mtDNA)种间保守序列,设计合成了3对特异性引物与TaqMan探针,通过对荧光PCR反应体系和反应条件的优化筛选,建立了三重荧光PCR方法,在同一个荧光PCR反应中完成3种动物源性成分的检测。用该方法对16种不同源性的动物DNA进行检测,结果表明能特异地鉴别检测出牛、山羊和绵羊源性成分,且敏感性比现行国标PCR法高100倍。该方法适用于饲料、肉制品、奶制品等动物源性产品的检测。In this study, a multiplex fluorescent PCR assay capable of detecting and differentiating bovine, goat and sheep-derived materi- als was developed using specific primers and TaqMan probes designed according to mitochondrial DNA gene sequences. The multiplex fluo- rescent PCR reaction system was optimized. The developed method could successfully detect bovine, goat and sheep-derived materials of 16 animals. The result showed that the real-time PCR can work accurately and efficiently, and can be 100 times as sensitive as conventional PCR of GB/T 20190-2006. Therefore, this method has the potential application for detection of bovine, goat and sheep-derived materials in feed, meat products, milk products, etc.

关 键 词：线粒体DNA 荧光PCR 牛 山羊和绵羊源性成分 

分 类 号：S816.17[农业科学—饲料科学]