贵州黑山羊独立生长因子1B基因cDNA克隆与生物信息学分析  

Cloning and bioinformatics analysis of GFI1B gene cDNA in the Guizhou Black Goat

作　　者：陈志[1] 罗卫星[1] 刘若余[1] 石照应[1] 张依裕[1] 蔡惠芬[1] 谈为忠[2] 

机构地区：[1]贵州大学动物科学学院,高原山地动物遗传育种与繁殖教育部重点实验室,贵州贵阳550025 [2]苏州市吴中区金庭动物防疫站,江苏苏州215111

出　　处：《畜牧与兽医》2012年第7期27-32,共6页Animal Husbandry & Veterinary Medicine

基　　金：贵州省农业科技攻关项目[黔科合NY字(2008)3044]

摘　　要：选择与羊同源性较高的牛独立生长因子1B(GFI1B)基因序列设计特异性引物,提取贵州黑山羊脾脏总RNA,通过RT-PCR技术对GFI1B基因进行克隆测序及序列分析。结果表明:首次克隆了贵州黑山羊GFI1B基因cDNA序列996 bp,GenBank登录号为JN662390,编码331个氨基酸。贵州黑山羊GFI1B基因与牛的同源性高达97.0%。聚类分析显示:哺乳动物、禽类和两栖类各为一类。生物信息学分析表明:山羊GFI1B分子包括1个低组分复杂性区域、6个锌指结构域ZnF-C4。同时,预测结果还表明GFI1B分子不包含信号肽序列且没有跨膜螺旋结构域,26个磷酸化位点,蛋白糖基化位点有7个。Cow GF11B genome sequence highly homologinized with that of sheep was selected for design a specific primer. Total RNA was extracted from the Guizhou Black Goat of spleen and the eDNA encoding GF11B was obtained by the reverse transcription PCR （RT-PCR）. The purified RT-PCR product was cloned into T vector and the sequence was analyzed. The results demonstrated that the 996bp product was found in the Guizhou Black Goat GFI1B eDNA, and GenBank accession number were JN662390, respectively. It encoded 331 amino acids. GFI1B protein in the Guizhou Black Goat had 97. 0% homology with cow. Cluster analysis revealed that probably mammals, birds and amphibians, each of these belonged to different categories, separately. Bioinformatics analysis indicated that goat GFll B protein contained one low compositional complexity region, six ZnF-CA without signal peptide, transmembrane helix, 26 phosphorylation sites and 7 prodiction O- glycosylation sites.

关 键 词：贵州黑山羊 GFI1B 克隆 序列分析 

分 类 号：S813.2[农业科学—畜牧学]