左旋棉酚通过细胞自噬下调Namalwa细胞中B淋巴细胞刺激因子的表达  

Mechanism of(-)-gossypol down-regulating B lymphocyte stimulator expression in Namalwa cells via autophagy

作　　者：丁雯[1] 倪振洪[2] 程攀科[2] 王滨[2] 黄刚[2] 何凤田[2] 连继勤[2] 徐兴然[1] 

机构地区：[1]西南大学药学院,重庆400716 [2]第三军医大学基础医学部生物化学与分子生物学教研室,重庆400038

出　　处：《第三军医大学学报》2012年第16期1613-1616,共4页Journal of Third Military Medical University

基　　金：重庆市自然科学基金(CSTC2011BB5030)~~

摘　　要：目的探讨左旋棉酚[(-)-gossypol]下调Namalwa细胞中B淋巴细胞刺激因子(B lymphocyte stimulator,BLyS)的可能机制。方法采用CCK8、台盼蓝活细胞染色检测左旋棉酚在体外对Namalwa细胞的生长抑制作用,吖啶橙(acridine orange,AO)染色观察经左旋棉酚处理后细胞自噬的形态特征,Western blot检测细胞中BLyS和自噬蛋白微管蛋白轻链3(LC3)的表达情况。结果左旋棉酚对Namalwa细胞有明显的增殖抑制作用,并且具有时间和剂量依赖性;AO染色后细胞内可观察到明显的细胞自噬小体;Western blot结果显示,左旋棉酚可下调Namalwa细胞中BLyS的表达,并上调LC3Ⅱ蛋白的表达;自噬特异性抑制剂3-methyladenine(3-MA)可显著抑制左旋棉酚对BLyS的下调与LC3Ⅱ的上调作用。结论左旋棉酚可能通过诱导细胞自噬来下调B淋巴细胞中BLyS的表达,从而抑制细胞生长。Objective To explore the mechanism of （-）-gossypol down-regulating B lymphocyte stimulator （BLyS） expression in human B-cell lymphoma cell line Namalwa. Methods The growth inhibition of Namalwa ceils by （-）-gossypol was detected by CCK8 （cell counting kit-8） kit and trypan blue staining. Cell autophagy was analyzed by observing the autophagosome formation by acridine orange （AO） staining. The ex- pression levels of BLyS and LC3 were detected by Western blotting. Results The growth of Namalwa cells was significantly inhibited by （-）-gossypol in a time and dose-dependent manner. After the （-）-gossypol treat- ment, the amount of autophagosomes dramatically increased. The expression level of LC3 II was up-regulated and the expression level of BLyS was down-regulated in Namalwa ceils after the treatment with （-）-gossypol, and this effect could be significantly attenuated by 3-methyladenine （3-MA）. Conclusion （-）-gossypol may inhibit tile growth of B lymphocytes through autophagy-medicated BLyS down-regulation.

关 键 词：B淋巴细胞 左旋棉酚 自噬 B淋巴细胞刺激因子 

分 类 号：R392.11[医药卫生—免疫学] R967[医药卫生—基础医学]