TNF-α、IL-8与急性重症胰腺炎肺损伤中PMNs凋亡延迟的相关性研究  

Correlation of TNF-α and IL-8 with delayed apoptosis in polymorphonuclear leukocytes in rats with severe acute pancreatitis-associated acute lung injury

作　　者：徐军[1] 张东[1] 仵正[1] 刘学民[1] 马清涌[1] 潘承恩[1] 

机构地区：[1]西安交通大学医学院第一附属医院肝胆外科,西安710061

出　　处：《第三军医大学学报》2012年第16期1654-1657,共4页Journal of Third Military Medical University

摘　　要：目的探讨肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)在急性重症胰腺炎(acute severe pancreatitis,ASP)合并急性肺损伤中中性粒细胞(polymorphonuclear leukocyte,PMNs)凋亡延迟的相关性及其机制。方法选取雄性SD大鼠48只,穿刺胆胰管并注入3.5%牛磺胆酸钠制作ASP合并急性肺损伤大鼠模型,分为牛磺胆酸钠注射后3、6、12 h及对照组。病理检测胰腺及肺组织;于预定时相取支气管灌洗液,密度梯度离心分离PMNs,流式细胞仪测定PMNs凋亡、坏死比例;ELISA检测灌洗液中TNF-α及IL-8的含量;检测NF-κB在肺组织中的表达。结果 ALI大鼠肺灌洗液中PMNs凋亡、坏死比例增加,凋亡延迟。支气管-肺泡灌洗液中的TNF-α及IL-8的含量较对照组显著增加,灌洗液中细胞TNF-α及IL-8 mRNA表达增加,不同干预时间组间具有显著差异,具有时间依赖效应;肺组织中NF-κB的表达显著增加。结论TNF-α、IL-8在ASP合并急性肺损伤中可能发挥一定的作用。Objective To determine the roles of TNF-α and IL-8 in the delayed apoptosis in polymorphonuelear leukocyte （PMNs） in rats with aeute lung injury after acute severe panereatitis （ASP）. Methods Twenty-four male SD rats were inflicted with 3.5% tauroeholie acid into the biliopanereatic duet to establish ASP rat model complicated with acute lung injury. After injection, these rats were divided into 3, 6, 12 h groups with 8 rats in each group, and another 24 health rats served as control. At scheduled time, bronehoalveolar lavage fluid from the model and control group was drawn and then PMNs were obtained by gradient density gradient centrifugation. Through flow eytometry, the ratio of apoptosis and necrosis of PMNs was assayed. The expression of NF-κB p65 was detected by immunohistochemical assay. The levels of TNF-α and IL-8 in the bronchoalveolar lavage fluid were determined by ELISA. Results The levels of TNF-α and IL-8 in the bronchoalveolar lavage fluid were significantly increased compared with the control and remained higher for a relatively long period （P 〈 0. 05 ）. The ratio of survival PMNs was increased （P 〈 0. 05 ）, indicating that apoptosis was delayed. The expression of NF-κB p65 in lung tissue in the model rats were significantly higher than control （P 〈 0. 05 ）. Conclusion TNF-α and IL-8 might play important roles in acute lung injury after acute severe pancreatitis.

关 键 词：急性重症胰腺炎 肺损伤 肿瘤坏死因子-α 白细胞介素-8 

分 类 号：R392.11[医药卫生—免疫学] R392.3[医药卫生—基础医学]