脉冲电磁场对人单核细胞激活的影响  

The Effects of PEMF on the Activation of Human Monocytes

作　　者：陈晓莹[1,2] 韩晓宇[1,2] 王谦[3,1] 吴文超[1] 刘小菁[1,2] 

机构地区：[1]四川大学华西医院心血管疾病研究室,成都610041 [2]四川大学华西医院心内科,成都610041 [3]四川大学华西医院康复医学科,成都610041

出　　处：《生物医学工程学杂志》2012年第4期604-608,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30870598;11072163)

摘　　要：研究不同参数的脉冲电磁场(PEMF)干预对人单核(THP-1)细胞激活的影响。将体外培养的THP-1细胞分别以频率为32Hz或64Hz、强度为1mT的PEMF进行干预,2次/d,30min/次,间隔8h,共3d,以频率为0Hz作为对照组。采用计数法观察PEMF处理后THP-1细胞与人脐静脉内皮细胞(HUVECs)之间的黏附变化;ELISA法检测THP-1细胞培养基中单核细胞趋化因子1(MCP-1)的分泌情况;荧光定量PCR法观察THP-1细胞MCP-1基因表达改变。结果观察到,PEMF干预能明显抑制THP-1细胞与HUVECs间黏附,降低THP-1细胞MCP-1基因表达及其蛋白分泌水平。本实验提示,频率为32Hz或64Hz、场强为1mT的PEMF干预3d后,能明显抑制THP-1细胞激活。The aim of the present study is to investigate the effect of pulsed electromagnetic field （PEMF） on the acti- vation of human monocytes （THP-1）. Cultured THP-1 cells were exposed to PEMF stimulation with radiation of 32Hz or 64Hz respectively, using sinusoidal wave, and linT, twice a day, 30 minutes each time, with an interval of 8 hours, for 3 days. Those with 0Hz stimulation served as the controls. Monocytes activation was monitored by measuring both the release of monocyte chemoattractant protein-1 （MCP-1） from monoeytes and their adhesion to monolayers of human umbilical vein endothelial cells （HUVECs）. The adhesion of THP-1 cells to HUVECs was e- valuated by cell counting method. The secretion of MCP-1 from THP-1 cells was detected by ELISA and MCP-1 mRNA expression was assessed by real time quantitative RT-PCR. The data showed that exposure to PEMF with a- bove parameters could significantly inhibit the adhesion of THP-1 cells to HUVECs and decrease the MCP-1 mRNA and protein expression. The results demonstrated that exposure to PEMF of lmT, 32Hz or 64Hz for 3 days could significantly inhibit the activation of THP-1 cells.

关 键 词：脉冲电磁场 单核细胞 激活 单核细胞趋化因子1 

分 类 号：R580[医药卫生—内分泌]