尾吊模型模拟微重力抑制小鼠卵母细胞的成熟  

Tail-suspended Model Simulating Mouse Oocytes Maturation Inhibited with Microgravity

作　　者：吴嫦丽[1,2] 李莉[1] 卫恒习[1] 吴珍芳[1] 江青艳[1] 张守全[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642 [2]广东医学院基础医学院病理教研室,湛江524023

出　　处：《生物医学工程学杂志》2012年第4期687-690,696,共5页Journal of Biomedical Engineering

基　　金：973计划项目资助(2011CB944202;2011CB944203;2010CB945001);科技支撑计划项目资助(2011BAD19B03)

摘　　要：本文研究模拟微重力对小鼠卵母细胞成熟的影响,探讨尾吊模型模拟微重力研究雌性小鼠生殖功能的可行性。采用转壁式生物反应器模拟微重力体外培养小鼠卵母细胞,以及对小鼠进行旋转和尾吊处理,观察小鼠卵母细胞的成熟情况。结果表明:体外旋转培养和尾吊模型产生的模拟微重力条件下,小鼠卵母细胞的成熟受到抑制,异常率增加。表现为体外旋转培养小鼠卵母细胞的成熟率为8.93%,显著低于1g重力条件下的72.33%;尾吊模型小鼠卵母细胞的成熟率为14.54%,显著低于对照组的73.29%。与对照组比较,小鼠旋转模型对小鼠卵母细胞的成熟无明显影响,提示该旋转模型不适用于模拟微重力的研究。尾吊模型可适用于地面研究模拟微重力对母鼠繁殖机能的影响。We studied the effects of simulated microgravity on mouse oocytes maturation, and analyzed whether the tail-suspended model can be applied to investigate simulated microgravity effects on reproductive processes in female mice. Mouse oocytes were cultured in vitro with microgravity simulated by a rotating wall vessel bioreactor and by tail-suspended model, and the maturation rate of the mouse ooeytes in the two models were examined in vivo. The maturation rate of mouse ooeytes cultured in simulated microgravity was 8.93%, and that was 72.33% in lg gravi- ty. In vivo, oocyte maturation rate had no significant difference between the rotational group and control group. Mi- crogravity simulated by the tail-suspended model inhibited mouse oocytes maturation and increased the rate of oocytes abnormity. The maturation rate of tail-suspended mouse oocytes was 14.54%, which was significantly lower than that of control group. Tail-suspended model should be an ideal model to investigate simulated microgravity effects on reproductive processes of female mice.

关 键 词：尾吊模型 模拟微重力 卵母细胞 

分 类 号：Q813.1[生物学—生物工程] Q852.22