失血性休克过程中红细胞携氧-释氧动力学研究  

Kinetics of Carrying and Releasing Oxygen of Red Blood Cells in Hemorrhagic Shock

作　　者：兰珂[1] 王翔[1] 

机构地区：[1]重庆大学生物工程学院,教育部生物流变科学与技术重点实验室,重庆400044

出　　处：《生物医学工程学杂志》2012年第4期701-704,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(10572159);重庆市科委科技计划项目攻关项目(cstc,2006ba5010);高等学校学科创新引智计划资助项目(B06023);中央高校基本科研业务费资助项目(CDJXS10231121)

摘　　要：失血性休克过程中,红细胞自身代谢与功能的变化会导致红细胞携氧-释氧能力的改变。本文对不同休克程度红细胞携氧-释氧能力的变化进行了监测,探讨失血性休克的危重程度与红细胞携氧-释氧能力之间的相互关系,并进行生理生化指标分析。本文采用健康SD大鼠20只(雌雄各10只),建立失血性休克模型,分别在初始、失血20%、30%和40%时采集红细胞,并利用血氧分析仪测定红细胞携氧-释氧热力学参数P50,效应时间Tc50、Tr50,同时检测了2,3-DPG、pH值、葡萄糖和乳酸脱氢酶的变化。实验结果表明:随着休克程度的加深,P50值不断增大,乳酸脱氢酶增加,而Tr50、pH值、葡萄糖均呈现减小的趋势,Tc50变化不明显;红细胞携氧能力相对稳定,而向组织释氧的能力仅略有增加,机体氧耗无法得到满足导致多器官衰竭。This paper focuses on the capacity changes of the red blood cells carrying and releasing oxygen. In order to provide theoretical basis and guidance for the clinical treatment of uncontrolled hemorrhagic shock, we investigated cases in the occurrence of uncontrolled hemorrhagic shock, and also analyzed the mechanism of physiological cause. Twenty healthy SD rats were used to build the hemorrhagic shock model. Red blood cells were collected at the initial step, at blood lose 20%, 30%, and 40%to determine the red blood cells capacities of carrying and releasing oxygen. The Hemox-analyzer was used to measure the thermodynamic parameters of the P50, the kinetic parameters of Tc50 and Tr50. The 2, 3-DPG, pH value, glucose and lactate dehydrogenase changes were also captured and recorded. With the aggravation of shock, P50 and lactate dehydrogenase are continuously increased, Tr50, pH value and glucose are tended to reduce significantly, and Tc50 does not change significantly. With the increase of blood loss, red blood cell capacity to carry oxygen is stable, and the ability to release oxygen is increased, so that the oxygen consumption cannot be satisfied, which causes organ failure.

关 键 词：失血休克 红细胞 Tc50 Tr50 2 3-二磷酸甘油酸 

分 类 号：R459.7[医药卫生—急诊医学]