仙珍骨宝干预泼尼松诱发大鼠的骨丢失  

Effects of Xianzhengubao on bone loss in prednisone-treated rats

作　　者：许碧莲[1] 陈广斌[2] 陈文双[1] 张新乐[1] 吴铁[1] 崔燎[1] 

机构地区：[1]广东医学院药理教研室,广东省湛江市524023 [2]深圳市龙岗区人民医院,广东省深圳市518172

出　　处：《中国组织工程研究》2012年第28期5136-5140,共5页Chinese Journal of Tissue Engineering Research

基　　金：东莞市科技计划项目(2008108101047);课题名称为"仙珍骨宝抗骨质疏松有效部位群的筛选";深圳市龙岗区科技计划项目(YL-2009124);课题名称为"仙珍骨宝抗骨质疏松有效部位群的筛选"~~

摘　　要：背景:仙珍骨宝胶囊由蛇床子、淫羊藿等制成,对糖皮质激素、维甲酸等诱发的骨质疏松有一定的预防作用。目的:建立泼尼松诱发大鼠骨质疏松模型,通过骨形态计量学和骨生物力学等方法探讨仙珍骨宝对大鼠不同部位骨骼的影响。方法:32只SD大鼠随机分成对照组、模型组和仙珍骨宝高、低剂量组。后3组给予泼尼松3.5mg/(kgd)灌胃建立骨质疏松模型。仙珍骨宝高、低剂量组大鼠在泼尼松灌胃1h后给予仙珍骨宝胶囊0.95g/(kgd)、0.475g/(kgd)。结果与结论:与对照组比较,模型组大鼠股骨骨密度、弹性载荷、最大载荷和断裂载荷均下降,胫骨上段动态参数标记周长百分数、骨表面形成率、骨转换率均降低,但静态参数骨小梁面积百分数、骨小梁数量、骨小梁厚度和骨小梁分离度无明显变化。胫骨中段皮质骨形态计量学参数均无明显变化。与模型组比较,仙珍骨宝高剂量组股骨密度、骨生物力学参数,胫骨上段标记周长百分数、骨表面形成率和骨转换率增加,但胫骨中段的骨形态计量学参数无明显变化。与模型组比较,仙珍骨宝低剂量组各项参数均无明显变化。提示高剂量仙珍骨宝促进骨形成,可预防泼尼松所致大鼠股骨骨密度及生物力学的降低,但对胫骨中段皮质骨无明显影响。BACKGROUND： Xianzhengubao capsule made from Cnidium, Epimedium and others has a certain protective role in glucocorticoid and retinoic acid-induced osteoporosis. OBJECTIVE： To study the effect of Xianzhengubao on different bones in prednisone-treated rats by bone histomorphometry and bone biomechanics methods. METHODS： Thirty-two Sprague-Dawley rats were randomly divided into control, prednisone, high- and low-dose Xianzhengubao groups. Rats in the latter groups were given prednisone at 3.5 mg/kgod first to establish osteoporosis models. High- and low-dose Xianzhengubao groups were administrated with Xianzhengubao capsule at 0.95 g/kg·d and 0.475 g/kg·d, respectively, after prednisone treatment. RESULTS AND CONCLUSION： Compared with the control group, bone mineral density and biomechanical properties （elastic load, maximum load, break load） of the femur were significantly decreased in the prednisone group. Percent labeled perimeter, bone formation rate per bone surface and per unit of bone volume of the proximal tibial metaphysis were decreased in the prednisone group, but there was no significant difference both in static parameters of proximal tibial metaphysis and in tibial shaft. Compared with the prednisone group, bone mineral density and biomechanical properties of the femur, percent labeled perimeter and bone formation rate per bone surfaceof the proximal tibial metaphysis were increased, but there was no significant change in tibial shaft in the high-dose Xianzhengubao group. Compared with the prednisone group, there was no significant change in the low-dose Xianzhengubao group. The results demonstrates the Xianzhengubao can promote bone formation and prevent the decrease of bone mineral density and biomechanical properties of the femur in prednisone-treated rats, but has no effect on the cortical bone of the tibial shaft.

关 键 词：仙珍骨宝 骨质疏松 泼尼松 骨密度 骨生物力学:骨形态计量学 组织构建 

分 类 号：R318[医药卫生—生物医学工程]