低功率激光修复末端病大鼠跟腱胶原  

Low level laser for repair of collagen in the tendon of rats with enthesiopathy

作　　者：沈勇伟[1] 史晓伟[2] 

机构地区：[1]苏州大学体育学院,江苏省苏州市215021 [2]海南师范大学体育系,海南省海口市570100

出　　处：《中国组织工程研究》2012年第28期5165-5169,共5页Chinese Journal of Tissue Engineering Research

基　　金：江苏省社会发展项目(BS2006020);课题名称:运动训练预防跟腱损伤的研究~~

摘　　要：背景:低功率激光疗法能对生物体产生特定的刺激作用(光生物调节作用),一般在不引起温度上升的情况下能引起某些生物学效应。目的:观察低功率激光对末端病大鼠跟腱组织中羟脯氨酸含量、Ⅰ型胶原基因表达的影响。方法:将96只Wistar大鼠随机分组:正常对照组不进行任何干预;造模组以电刺激跳跃法刺激大鼠足部,建立末端病大鼠模型;激光照射组建立末端病大鼠模型后于跟腱处给予低功率激光照射治疗。结果与结论:与正常对照组比较,末端病模型大鼠跟腱组织中羟脯氨酸含量与Ⅰ型胶原水平明显下降(P<0.05)。经低功率激光照射治疗后,末端病模型大鼠跟腱羟脯氨酸含量增加,Ⅰ型胶原合成在转录水平上的表达升高,且这种作用在造模7d后开始出现,14d时效果非常明显。表明低功率激光可以提高末端病模型大鼠羟脯氨酸合成量,促进胶原的代谢,在跟腱早期愈合中有一定的促进作用。BACKGROUND： Low level laser therapy （LLLT） has the specific stimulation effect on organism （photobiomodulation）. In general case, it can produce some biological effects without temperature increase. OBJECTIVE： To observed the effect of low level laser on contents of hydroxyproline and gene expression of collagen Ⅰ in the tendon of rats with enthesiopathy. METHODS： Ninety-six male Wistar rats were randomly divided into two groups. The control group was without ant treatment. Rats in the model group were stimulated to the feet with electrostimulating jumping method to build the mode of enthesiopathy. Rats in the LLLT group were treated with LLLT to the tendon after model building. RESULTS AND CONCLUSION： Compared with the control group, the contents of hydroxyproline and gene expression of collagen ] in the tendon of rats with enthesiopathy were significantly decreased （P 〈 0.05）. After LLLT, low level laser could increase the contents of hydroxyproline and gene expression of collagen I in the tendon of rats with enthesiopathy, and this started from 7 days and was obviously increased at 14 days. It is indicated that low level laser can improve the contents of hydroxyproline and the metabolism of collagen ] in the tendon of rats with enthesiopathy, and can promote the healing of tendon in early stage.

关 键 词：低功率激光 末端病 跟腱 羟脯氨酸 Ⅰ型胶原mRNA 

分 类 号：R318[医药卫生—生物医学工程]