TGF-β1抗体复合生物蛋白胶预防鞘管区屈肌腱粘连的组织学观察  

The preventing effect of the compound of transforming growth factor-β1 antibody with fibrin glue(TGFβ_1 Ab+FG) on postoperative adhesions in the chicken's flexor tendon in zone Ⅱ(no man's land)

作　　者：窦永峰[1] 房清敏[1] 王志刚[1] 孙兆忠[1] 仲江波[1] 

机构地区：[1]山东滨州医学院附属医院骨科,256603

出　　处：《中国矫形外科杂志》2012年第16期1489-1492,共4页Orthopedic Journal of China

基　　金：山东省卫生厅计划基金资助项目(编号:2003CA1CFB1)

摘　　要：[目的]研究TGF-β1抗体(transforming growth factor-β1 antibody,TGF-β1Ab)复合生物蛋白胶(fi-brin glue,FG)预防鞘管区屈肌腱粘连的作用和对肌腱愈合的影响。[方法]成年雄性来亨鸡72只随机平均分为4组,每组18只。左足第3趾趾深屈肌腱横断,四股交叉缝合肌腱,鞘管未缝合。按鞘管区给药分为4组:A组TGF-β1Ab、B组FG、C组TGF-β1Ab+FG、D组生理盐水。仅术中注入一次药物。术后1、3、8周,每组各取6只鸡第3趾行大体及组织学观测。[结果]大体观察:术后1、3、8周肌腱粘连程度分级A组与B组无统计学差异(P>0.05),C组与其余3组比较有统计学差异(P<0.05)。组织学观测:术后3、8周A、B、D组胶原纤维排列紊乱,C组胶原纤维排列整齐。[结论]TGFβ1抗体复合生物蛋白胶可以有效预防术后肌腱粘连,不影响肌腱的正常愈合进程。[ Objective ] To explore the preventing effects of the compound of transforming growth factor-β1 and fibrin glue （TGFβ1 Ab ＋ FG） on prevention of peritendinous adhesive formation. [ Method] Seventy-two Leghorn chickens were random- ly divided into 4 groups （groups A, B,C and D）, 18 chickens for each group, and the long flexor tendons of the 3rd toes in zone Ⅱ of 72 leghorn chickens were transversed and sutured with the 4-strand cruciate repair technique to make defect models. In group A, TGFβ1 antibody was applied at repair site. In group B, FG was applied at repair site. In group C, the TGF-β1 Ab and FG O. 2 ml compound was applied at repair site. In group D, normal sodium was applied at repair site. At 1, 3 and 8 weeks after operation, the tendons of 6 chickens in each group were harvested for morphological and histological evaluation. [ Result] The gross observation showed that the differences of grading of tendon adhesion were not significant among groups A, B, and D, but in group C, the differences were significant between A, B, and D, 3 and 8 weeks after operation （P 〉 O. 05）. Histological observation showed that collagen fibers of group A, B and D were in a great disorder, but were well-formed in group C. [ Conclusion] The compound of TGFβ1 antibody with FG could significantly prohibit the formation of fibrous adhesions with- out interfering with the healing process.

关 键 词：肌腱损伤 肌腱粘连 TGF-Β1抗体 生物蛋白胶 鸡 

分 类 号：R687.2[医药卫生—骨科学]