猪小肠黏膜下层作为组织工程肌腱支架的强度  

Mechanical strength of porcine small intestinal submucosa used as a tissue-engineered tendon scaffold

作　　者：孙文才[1] 李玉成[1] 李博[1] 姜鑫[1] 李晓龙[1] 陈永春[1] 白玉江[1] 

机构地区：[1]齐齐哈尔医学院附属第三医院,黑龙江省齐齐哈尔市161000

出　　处：《中国组织工程研究》2012年第29期5361-5365,共5页Chinese Journal of Tissue Engineering Research

基　　金：黑龙江省教育厅资助项目(12511620)~~

摘　　要：背景:小肠黏膜下层是一种能够适合于细胞迁移、生长和增殖的良好的支架材料,已被应用于组织工程研究。目的:分析猪小肠黏膜下层作为组织工程肌腱支架的可行性。方法:将24只左腿屈趾肌腱缺损罗曼鸡随机分组,实验组于缺损处植入猪小肠黏膜下层与鸡胚趾深屈肌肌腱细胞复合物,对照组于缺损处植入单纯猪小肠黏膜下层,植入后3,6,9周检测两组植入材料肌腱最大拉伸强度。结果与结论:植入后第3周时,实验组和对照组植入材料肌腱最大拉伸强度差异无显著性意义。植入后第6,9周时,实验组植入材料肌腱最大拉伸强度高于对照组(P<0.05)。表明肌腱细胞-小肠黏膜下层材料复合构建的组织工程肌腱力学强度强于单纯小肠黏膜下层材料修复肌腱,可作为组织工程肌腱支架材料。BACKGROUND： Small intestinal submucosa is a good scaffold material for cell migration, growth and proliferation, which has been applied in tissue engineering research. OBJECTIVE： To analyze the feasibility of porcine small intestinal submucosa used as the tendon scaffold for tissue engineering METHODS： Totally 24 Roman chicken with flexor tendon defects of the left leg were randomly divided into experimental group and control group. The composite of porcine small intestinal submucosa and flexor digitorum profoundus tendon in chicken embryos were implanted into the defect area of chicken in the experimental group. The chicken in the control group were implanted with isolated porcine small intestinal submucosa to their defects. Maximum tensile strength of tendon materials in the two groups were detected at weeks 3, 6 and 9 after implantation. RESULTS AND CONCLUSION： At week 3 after implantation, in terms of the maximum tensile strength of tendon materials, there was no significant difference between the experimental group and control group. At weeks 6 and 9 after implantation, the maximum tensile strength of tendon materials in the experimental group was higher than that in the control group （P 〈 0.05）. These findings suggest that the mechanical strength of tissue-engineered tendon scaffolds composited by tendon cells and small intestinal submucosa is stronger than that of tendon repaired by the pure small intestinal submucosa. Small intestinal submucosa can be used as a tendon scaffold material for tissue engineering.

关 键 词：小肠黏膜下层 组织工程 肌腱 支架 肌腱缺损 生物材料 

分 类 号：R318[医药卫生—生物医学工程]