三种排龈线对体外培养人牙龈成纤维细胞毒性的比较  

Comparison of cytotoxicity among three gingival retraction cords on human gingival fibroblasts in vitro

作　　者：倪莹[1] 张晓明[2] 惠敏[1] 刘健 

机构地区：[1]滨州医学院口腔学院,山东省滨州市256603 [2]滨州医学院附属医院口腔修复科,山东省滨州市256603 [3]日照市中医院正畸科,山东省日照市276800

出　　处：《中国组织工程研究》2012年第29期5396-5400,共5页Chinese Journal of Tissue Engineering Research

基　　金：山东省教委课题(3120)~~

摘　　要：背景:近年来,国内外学者研究热点主要集中在排龈药物的排龈效果,而对商品化排龈线的生物相容性研究较少。目的:评价3种排龈线对人牙龈成纤维细胞的毒性作用,为指导临床选择理想的排龈线提供理论依据。方法:3种排龈线(含肾上腺素排龈线、含硫酸铝排龈线、不含药排龈线)浸透在含体积分数为10%胎牛血清的DMEM中24h,再按1∶1和1∶4浓度稀释,分别作用于体外培养的牙龈成纤维细胞,阴性对照组为含体积分数为10%胎牛血清的DMEM。结果与结论:MTT比色法结果显示,3种排龈线均可抑制人牙龈成纤维细胞生长及增殖,3种排龈线浸提液1∶1稀释后的毒性明显大于1∶4稀释组和对照组(P<0.05),肾上腺素排龈线浸提液1∶4稀释后的毒性也明显大于对照组(P<0.05)。透射电镜观察显示肾上腺素组部分细胞出现凋亡。结果可见3种排龈线对牙龈成纤维细胞都有一定的毒性,肾上腺素排龈线毒性最大,硫酸铝排龈线毒性次之,不含药排龈线毒性最小。BACKGROUND： In recent years, the gingival retraction effect of gingival retraction drugs is a hot topic for domestic and foreign scholars, while the biocompatibility of the commercial gingival retraction cord is less involved. OBJECTIVE： To evaluate the cytotoxicity effect of three kinds of gingival retraction cords on human gingival fibroblasts and to provide theoretical basis for choosing proper gingival retraction cords in clinic. METHODS： Three kinds of gingival retraction cords （epinephrine-impregnated cord, aluminium sulphate-impregnated cord and non-drug-impregnated cord） were incubated in Dulbecco＇s modified Eagle＇s medium containing 10% fetal bovine serum for 24 hours, according to 1：1 and 1：4 dilution, and then the cords acted on gingival fibroblasts cultured in vitro. Dulbecco＇s modified Eagle＇s medium containing 10% fetal bovine serum was used as negative control group. RESULTS AND CONCLUSION： The results of MTT assay demonstrated that the three kinds of gingival retraction cords all could inhibit human gingival fibroblasts growth and proliferation. The cytotoxicity of the three kinds of gingival retraction cords in the leaching liquor with 1：1 dilution was significantly higher than that of 1：4 dilution and control groups （P 〈 0.05）. The cytotoxicity of epinephrine-impregnated cord in the leaching liquor with 1：4 dilution was significantly higher than that of the control group （P 〈 0.05）. The results of transmission electron microscope showed that some cells in the epinephrine-impregnated cord group were found apoptosis. These findings suggest that the three kinds of gingival retraction cords all have certain cytotoxicity to gingival fibroblasts. The cytotoxicity of epinephrine-impregnated cord is the biggest, followed by aluminium sulphate-impregnated cord, and the cytotoxicity of the non-drug-impregnated cord is the smallest.

关 键 词：排龈线 牙龈成纤维细胞 细胞毒性 肾上腺素 硫酸铝 

分 类 号：R318[医药卫生—生物医学工程]