环孢素A对小鼠气管移植模型中气管上皮细胞的影响  

Effects of cyclosporine A on epithelial cells in a mouse trachea transplantation model

作　　者：李超[1] 鲍方[1] 王林毛[1] 郑卉[1] 陈昶[1] 高文[1] 

机构地区：[1]上海市肺科医院胸外科,上海市200433

出　　处：《中国组织工程研究》2012年第31期5777-5782,共6页Chinese Journal of Tissue Engineering Research

基　　金：上海市科委资助项目(9411963300)~~

摘　　要：背景:气管上皮的再生及完整性对预防肺移植后闭塞性细支气管炎起着最为关键的作用。目的:观察环孢素A对小鼠气管异位移植模型中移植气管上皮细胞增殖的影响。方法:将BALB/c小鼠气管异位移植到C57BL/6小鼠颈背部皮下,建立异位气管移植模型,将受体小鼠随机分组:实验组腹腔注射环孢素A25mg/(kgd),对照组不注射环孢素A。结果与结论:移植气管病理检查显示,环孢素A具有延缓上皮细胞剥离、减少淋巴细胞浸润以及延缓纤维化过程的作用,但最终仍无法阻止移植物管腔闭塞的发生。上皮细胞定量分析显示,环孢素A可以延缓上皮纤毛细胞下降幅度,延缓上皮细胞增殖高峰期,加快上皮细胞凋亡高峰期。细胞因子检测显示,环孢素A对白细胞介素4分泌具有促进作用,对干扰素γ分泌具有早期促进,中晚期抑制作用。BACKGROUND ： The regeneration and integrity of tracheal epithelial plays a key role in the prevention of bronchiolitis obliterans after lung transplantation. OBJECTIVE： To study the effects of cyclosporine A administration on the proliferation of epithelial cells in heterotopic transplanted trachea models. MEHODS： Tracheas from BALB/c mice were heterotopically transplanted to C57BL/6 mice to establish the heterotopic transplanted trachea model. The recipient mice were randomly divided, recipient mice in the experimental group were received 25mg/（kg·d） cyclosporine A injection, while the rest recipients in the control group did not have cyclosporine A injection. RESULTS AND CONCLUSION： The pathological examination of transplanted trachea showed that cyclosporine A could delay the progress of epithelial cells＇ stripping and fibrosis and depress the lymphocyte infiltration in heterotopic tracheal transplantation model. However, cyclosporine A did not prevent the final occurrence of graft obliteration. Quantitative analysis showed that cyclosporine A could slow down the decreasing process of epithelial ciliated cell and the proliferation peaks of epithelial cells and accelerate the apoptosis peaks of epithelial cells, Cytokines measurement showed that cyclosporine A could promote the secretion of interleukin-4 and interferon gamma γ in the early days after transplantation, and it could inhibit the secretion in the middle and advanced stage.

关 键 词：气管移植动物模型 环孢素A 上皮细胞 闭塞性气道疾病 小鼠 

分 类 号：R617[医药卫生—外科学]