硫化氢增加肝脏缺血再灌注损伤模型大鼠胰岛素样生长因子Ⅰ、核转录因子κB、肿瘤坏死因子α的表达  

Hydrogen sulfide increases the expression of insulin-like growth factor Ⅰ,nuclear factor kappa B and tumor necrosis factor alpha in a rat hepatic ischemia-reperfusion injury model

作　　者：王海久[1] 任利[1] 邓勇[1] 王聪[1] 李衍飞[1] 阳丹才让 樊海宁[1] 

机构地区：[1]青海大学附属医院肝胆胰外科,青海省西宁市810001

出　　处：《中国组织工程研究》2012年第31期5823-5827,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:硫化氢气体是一种新型气体信号分子,在生理浓度下具有抑制Ca2+内流、开放KATP通道功能,氧化还原等明确特定的作用。目的:观察硫化氢对大鼠肝脏缺血再灌注损伤胰岛素样生长因子Ⅰ、核转录因子κB及肿瘤坏死因子α表达水平的影响方法:70只SD大鼠随机等分为假手术组、缺血20min组,缺血20min+灌注2,4h组、硫氢化钠+缺血20min组、硫氢化钠+缺血20min再灌注2,4h组。除假手术组外,其余各组大鼠通过Pringle法建立大鼠全肝缺血再灌注模型,在术前5d中每天及术中向硫氢化钠+缺血20min组、硫氢化钠+缺血20min再灌注2,4h组,大鼠腹腔注射56μmol/kg硫氢化钠。结果与结论:肝脏缺血大鼠肝组织中胰岛素样生长因子Ⅰ、核转录因子κB和肿瘤坏死因子α表达明显下降(P<0.05),与缺血组比较,大鼠再灌注和腹腔注射硫化氢均能增加大鼠肝组织中胰岛素样生长因子Ⅰ、硫化氢、核转录因子κB和肿瘤坏死因子α的表达(P<0.05)。提示全肝缺血再灌注后可造成肝脏损伤,硫化氢增加肝脏缺血再灌注损伤模型大鼠胰岛素样生长因子Ⅰ、核转录因子κB、肿瘤坏死因子α的表达,肝脏损伤具有保护作用。BACKGROUND： Hydrogen sulfide is a novel gaseous signal molecule, which can inhibit Ca2＋ influx, open KATP channel and balance oxidation-reduction and exert other specific functions at physiological concentrations.OBJECTIVE： To observe the effect of hydrogen sulfide on the expression of insulin-like growth factor Ⅰ , nuclear factor κB and tumor necrosis factor a in the rat hepatic ischemia-reperfusion injury model.METHODS： Seventy SD rats were randomly divided into seven groups： sham operation, 20-minute ischemia group, 20-minute ischemia＋2 and 4 hours reperfusion group, sodium hydrosulfide＋20-minute ischemia group, and sodium hydrosulfide＋20-minute ischemia＋2 and 4 hours reperfusion group. Hepatic ischemia-reperfusion model were established by Pringle method for each group except the sham operation group. Sodium hydrosulfide＋20-minute ischemia group and sodium hydrosulfide＋20-minute ischemia＋2 and 4 hours reperfusion group were intraperitoneally injected with 56 pmol/kg sodium hydrosulfide each day for the 5 days before surgery.RESULTS AND CONCLUSION： The expression of insulin-like growth factor Ⅰ , nuclear factor κB and tumor necrosis factor a were significantly decreased in the ischemic rat liver tissue （P 〈 0.05）; in comparison with the ischemia groups, both reperfusion and intraperitoneal injection of sodium hydrosulfide could significantly increase the expression of insulin-like growth factor Ⅰ, nuclear factor κB and tumor necrosis factor a （P 〈 0.05）. It suggests that reperfusion after whole-liver ischemia can cause liver damage, and sodium hydrosulfide can protect the liver by increasing the expression of insulin-like growth factor 1, nuclear factor κB and tumor necrosis factor a in rat hepatic ischemia-reperfusion injury model.

关 键 词：硫化氢 肝脏缺血再灌注损伤 胰岛素样生长因子Ⅰ 核转录因子ΚB 肿瘤坏死因子Α 

分 类 号：R318[医药卫生—生物医学工程]