正交试验法优选颈眩宁胶囊的提取工艺  

Orthogonal Design in Optimizing Extracting Technology of Jingxuanning Capsule by Orthogonal Test

作　　者：王文娜[1] 胡小祥[1] 李宁[1] 冯正腾[1] 李国成 阎松 张鹤鸣[1] 

机构地区：[1]华南师范大学生物光子学研究院激光生命科学研究所,中医药与光子技术三级实验室,暨激光生命科学教育部重点实验室中药及创新药物研究所,广东广州510631 [2]珠海安生医药有限公司,广东珠海519020

出　　处：《今日药学》2012年第6期353-356,共4页Pharmacy Today

摘　　要：目的考察颈眩宁胶囊中天麻的提取工艺。方法采用HPLC法测定天麻中天麻素的含量,以天麻素的含量和浸膏收率为指标,采用正交试验法,考察醇溶性成分的提取工艺。结果优选出最佳提取工艺为加10倍量65%乙醇,浸泡3 h,回流提取3次,每次2 h。结论优选所得工艺使天麻素的提取率高,稳定性好,方法简单可行,成本低,可为工业生产提供依据。Objective To optimize the extracting technology of Jingxuanning capsule. Methods HPLC was adopted to determine the gastrodine in rhizoma gastrodiae. The extraction technology of falcoholsoluble extractive ingredients was optimized by orthogonal test with the extract yield and the extracting yield of gastrodine as indexes. Results The extracting technology was to extract 3 times for 2 h each time with 10 fold amount of 65% alcohol. Conclusion The experimental results provide that the optimized technique is efficient and stable.

关 键 词：天麻 天麻素 醇提工艺 正交试验 

分 类 号：R927[医药卫生—药学]