肺泡巨噬细胞表面甘露糖受体在免疫抑制大鼠肺隐球菌感染中的实验研究  

Effect of mannose receptor of alveolar macrophage on cryptococcal pneumonia in immunodepressive rats

作　　者：徐楠[1] 肖琴 姚玮[1] 储伟芳[1] 肖晓庆[1] 顾菊林[3] 温海[3] 

机构地区：[1]上海市东方医院皮肤科,上海200120 [2]上海浦东医院皮肤科,上海200125 [3]上海长征医院皮肤科,上海200003

出　　处：《中国真菌学杂志》2012年第3期146-149,167,共5页Chinese Journal of Mycology

基　　金：国家自然科学基金(30972817)

摘　　要：目的探讨免疫抑制大鼠肺泡巨噬细胞(AM)表面表达的甘露糖受体在AM吞噬隐球菌过程中的作用。方法建立免疫抑制(FK506)大鼠模型,流式细胞法检测AM表面甘露糖受体mRNA的表达量,观察免疫缺陷对AM表面甘露糖受体表达的影响。将实验(FK506)组与对照(正常)组大鼠的AM分别与荧光标记的新生隐球菌共培养,观察AM对隐球菌的吞噬情况。结果成功建立免疫抑制大鼠模型,免疫抑制大鼠表达的甘露糖受体与正常大鼠无统计学差别,对隐球菌的吞噬亦无差别。结论免疫抑制状态不是影响甘露糖受体表达的主要因素。Objective To explore the effect of mannose receptor of alveolar macrophage on cryptococcal pneumonia in immunodepressive rats.Methods Immunodepressive rats model was set up to observe the influence of immunodepression on the expression of mannose receptors by flow cytometry.The alveolar macrophages were cocultured with Cryptococcus neoformans and the phagocytosis was observered.Results On the endpoint of the observation, the expression of mannose receptors and the phagotosis of Cryptococcus neoformans in immunodepressive rats showed no significant difference with those in normal rats.Conclusions Immunodeficiency is not the key factor for the expression of mannose receptors.

关 键 词：肺泡巨噬细胞 甘露糖受体 隐球菌 免疫抑制 

分 类 号：R379.5[医药卫生—病原生物学]