正交试验优化梓醇的微波辅助提取工艺  

Optimization of Microwave-assisted Extraction Technology of Catalpol from Rehmannia Glutinosa Libosch by Orthogonal Method

作　　者：张秀丽[1] 于德红[2] 刘德胜[1] 张静[1] 

机构地区：[1]滨州医学院药学院,山东烟台264003 [2]河北联合大学药学院,河北唐山063000

出　　处：《现代生物医学进展》2012年第18期3487-3490,3537,共5页Progress in Modern Biomedicine

基　　金：国家自然基金项目(81102828);山东省自然基金(ZR2011HM011);山东省高等学校科技计划项目(J11LF-86);山东省高等学校优秀青年教师国内访问学者项目经费资助

摘　　要：目的:通过正交实验优选了地黄中梓醇的微波提取工艺。方法:以地黄粗提液中梓醇含量为指标,HPLC为含量测定方法,采用正交实验法,选取乙醇浓度(A)、料液比(B)、提取时间(C)和提取次数(D)4个因素,每个因素选取3个水平进行实验,确定了最佳提取工艺。结果:研究结果表明乙醇浓度为60%,料液比为4,微波提取3次,每次3 min为梓醇的最佳提取工艺。结论:微波辅助提取地黄中梓醇效率高,提取完全,方法可行。Objective： To optimize the microwave assisted extraction technology of catalpol from Rehmannia glutinosa Libosch. Methods： The content of catalpol in the extract was determined by high performance liquid chromatography （HPLC）. The orthogonal test table L9（34） was used with the factors of concentration of ethanol（A）, ratio of solvent to material（B）, extracting time（C） and extracting times （D）. Results： According to the results of the test, the optimum extraction conditions for catalpol were 60 % ethanol, material to liquid phase 4 w/v, 3 minutes and 3 times extraction. Conclusions： The extraction rate of catalpol is high and steady by this technology, which provides new evidence for further utilization.

关 键 词：地黄 梓醇 正交实验 微波提取 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]