艾灸干预不同穴位对束缚应激模型大鼠心理行为改变的影响  

Experimental Observation on Psychological and Behavioral Changes by Moxibustion at Different Acupoints in Model Rats of Restraint Stress

作　　者：孙洁[1] 魏惠芳[1] 仲大奎 温进[3] 曾霈君[1] 李滢[1] 杨磊[1] 张露芬[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]北京市房山区中医院康复科,北京102400 [3]北京门头沟区中医医院针灸科,北京102300

出　　处：《现代生物医学进展》2012年第19期3618-3623,共6页Progress in Modern Biomedicine

基　　金：北京市自然基金资助项目(7052037)

摘　　要：目的:观察艾灸不同穴位对束缚应激模型大鼠行为学改变的影响,进一步探讨艾灸对束缚应激所致心理行为改变的作用规律。方法:将35只雄性Wistar大鼠按分层随机法分为正常组、束缚应激模型组、艾灸百会(GV20)组、艾灸关元(CV4)组、艾灸足三里(ST36)组,每组各7只。除正常组外,余各组均采用自制布袋束缚大鼠30 min,每日1次,共20次,制备束缚应激大鼠模型,于造模第2天各治疗组捆绑固定后给予艾炷灸3壮,正常组及模型组捆绑束缚20 min,隔日一次,共10次。于造模前、造模后,治疗5次、10次分别采用高架十字迷宫检测各组大鼠开放臂进入次数比例(OE%)及开放臂停留时间比例(OT%)的变化。结果:①与造模前比较,艾灸百会(GV20)组大鼠造模后、治疗10次后OE%均降低明显,治疗5次后OT%升高明显,均有显著性差异(P<0.05),艾灸关元(CV4)组大鼠造模后OT%降低明显,有显著性差异(P<0.05),艾灸足三里(ST36)组大鼠造模后OE%和OT%、治疗10次后OE%均明显降低,均有显著性差异(分别为P<0.05,P<0.05,P<0.01);与造模刚结束比较,艾灸百会(GV20)组大鼠治疗5次后OE%与OT%均升高明显,均有显著性差异(P<0.05),艾灸关元(CV4)组大鼠治疗10次后OT%升高明显,有显著性差异(P<0.01),艾灸足三里(ST36)组大鼠治疗5次后OE%、治疗10次后OT%均明显升高,有显著性差异(分别为P<0.01,P<0.05);与治疗5次比较,艾灸百会(GV20)组、足三里(ST36)组大鼠治疗10次后OE%明显降低,艾灸关元(CV4)组大鼠治疗10次后OE%和OT%升高明显,均有显著性差异(P<0.05)。②与艾灸百会(GV20)组比较,艾灸关元(CV4)组与艾灸足三里(ST36)组大鼠在治疗5次、10次后OE%和OT%呈不同程度的升高或降低趋势,未见显著性差异(P>0.05)。结论:艾灸不同穴位可一定程度改善慢性束缚应激所致大鼠焦虑心理行为的变化;关元(CV4)、百会(GV20)、足三里(ST36)三穴均表现出一定抗焦虑效应,百会(GV20)穴在Objective： To observe the influence of moxibustion at different acupoints on behavioral changes caused by restraint stress and further discussion on regulation rule of moxibustion for psychological and behavioral changes caused by restraint stress.Meth-ods： According to the principle of stratified randomization the 35 male Wistar rats were divided into the normal group,the model group,Baihui group,Guanyuan group and Zusanli group,7 rats in each group.Except the normal group,the rest groups were tied by self-made bags for 30min each day,totally lasting 20 days to make the restraint stress model.From the 2nd day after model made,the treatment groups were treated by moxibustion for 3 cones on each acupoint,once every other day,a total of 10 times.All groups were respectively tested into the elevated plus-maze to detect the changes of the proportion of the number of entry into the elevated plus-maze open arms（OE%） and that of Residence time at the open arms（OT%） at four time points,including before and after modeling,after the 5th treatment and 10th treatment.Results： Compared with that in the groups before modeling,the OE % of the rats in Baihui group decre-ased significantly after modeling、after the 10th treatment and the OT% of the rats in Baihui group increased significantly after the 5th treatment（P〈0.05）,after modeling the OT % of the rats in Guanyuan group decreased significantly（P〈0.05）,after modeling the OE% and OT % of the rats,after the 10th treatment the OE % of the rats in Zusanli group all decreased significantly（P〈0.05,P〈0.05,P〈0.01）;Compared with that in the groups after modeling,after the 5th treatment the OE% and OT% of the rats in Baihui group both increased significantly（P〈 0.05）,after the 10th treatment the OT % of the rats in Guanyuan group increased significantly（P〈0.01）,the OE% of the rats in Zusanli group after the 5th treatment and the OT % of the rats in Zusanli group after the 10th treatment both increased significantly（P〈0.01,P〈0.05�

关 键 词：艾灸 束缚应激 高架十字迷宫 心理行为 

分 类 号：Q953[生物学—动物学] R245.97[医药卫生—针灸推拿学]