PQS抑制模拟缺血-再灌注心肌细胞FAS的表达  

Expression of FAS for saponin of Panax quinquefolius on cardiomyocytes by ischemia/reperfusion

作　　者：刘哲[1] 张峰[2] 吕岩红[3] 李丽阳[1] 崔玉东[1] 姚晓颖[2] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院 [2]黑龙江省大庆油田总医院 [3]哈尔滨医科大学

出　　处：《齐齐哈尔医学院学报》2012年第12期1552-1553,共2页Journal of Qiqihar Medical University

基　　金："黑龙江省青年科学基金"项目41411046-1-11014/C1202

摘　　要：目的探讨西洋参总皂苷(PQS)对缺血再灌注诱导心肌细胞凋亡的FAS mRNA的表达影响。方法利用原代培养的乳鼠心肌细胞模拟缺血模型2h,再恢复正常培养24h;RT-PCR及原位杂交检测PQS处理后FAS mRNA水平。结果 PQS能显著抑制缺血再灌注Fas的表达。结论实验表明PQS能抑制心肌细胞缺血再灌注FAS凋亡基因的表达。Objective To explore the effect of FAS mRNA for saponin of Panax quinquefolius（PQS） on cardiomyocytes after ischemia/reperfusion.Methods Myocardial was simulated by ischemic/reperfusion,FAS mRNA were detected by in situ hybridizati and RT-PCR.Results PQS can restrain the down-regulation of the Fas mRNA.Conclusions The data indicate that PQS can inhibit Fas mRNA on cardiomyocytes after ischemia/reperfusion.

关 键 词：PQS 缺血再灌注 心肌细胞 

分 类 号：R542.2[医药卫生—心血管疾病]