黄芪多糖对脂多糖诱导人THP21单核细胞MCP-1与IL-6表达的影响  

Effects of astragalus polysaccharide on the monocyte chemoattractant protein-1 and interleukin-6 expression induced by lipopolysaccharide in human THP21 mononuclear cells

作　　者：郭进强[1] 覃旻[1] 罗炳德[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院,广东广州510515

出　　处：《分子诊断与治疗杂志》2012年第4期258-261,共4页Journal of Molecular Diagnostics and Therapy

基　　金：国家自然科学基金-广东省联合基金(U0632009)

摘　　要：目的通过研究黄芪多糖(astragalus polysaccharide,APS)对脂多糖(lipopolysaccharide,LPS)诱导人单核细胞株THP21细胞趋化蛋白1(monocy techemoattractant protein1,MCP-1)及白介素6(interleukin-6,IL-6)的表达影响,探讨炎症状态下APS对单核细胞炎症因子表达的调控。方法体外培养人单核细胞THP21,以脂多糖刺激建立炎症细胞模型。以MTT法检测黄芪多糖对细胞的毒性,以荧光定量RT-PCR法检测黄芪多糖对THP21细胞MCP-1与IL-6mRNA水平表达的影响,以ELISA法检测黄芪多糖对THP21细胞MCP-1与IL-6细胞外分泌的影响。结果黄芪多糖在50、100、200μg/mL浓度下对THP21细胞均无明显毒性,100μg/mL的黄芪多糖明显抑制LPS诱导THP21细胞MCP-1与IL-6mRNA表达的增加及培养上清中MCP-1与IL-6的增加。结论黄芪多糖抑制脂多糖诱导的人单核细胞株THP21中MCP-1与IL-6的表达及分泌。Objective To study the effects of astragalus polysaccharide（APS） on the monocyte chemoattractant protein 1（MCP-1） and interleukin-6（IL-6） expression induced by lipopolysaccharide（LPS） in human mononuclear cells THP21, and the mechanism of APS in regulating the MCP-1 and IL-6 expression in monocytes in an inflammatory situation, Methods The human mononuclear cells THP21 were cultured and stimulated with LPS for 24 h. The effects of APS on the cytotoxicity were tested by MTT assay. The mRNA expression of MCP-1 and IL-6 was measured by real-time PCR. The MCP-1 and IL-6 in cytosolic extract were measured by ELISA. Results MTT assay showed that 5, 100, 200 μg/mL APS had no toxicity on the cell viability of THP21 cells. 100μg/mL APS inhibited the increase in mRNA expression and cytosolic extract of MCP-1 and IL-6 in THP21 cells induced by LPS, Conclusion The expression of MCP-1 and IL-6 induced by LPS was suppressed by APS in THP21 cells.

关 键 词：黄芪多糖 脂多糖 单核细胞趋化蛋白1 白介素6 

分 类 号：R285[医药卫生—中药学]