铁皮石斛微卫星SSR设计与应用  

Microsatellite Markers Primer Designing and Screening from Dendrobium officinale

作　　者：肖冬长[1] 张智俊[1] 管雨[1] 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,临安311300

出　　处：《生物技术通报》2012年第7期88-92,共5页Biotechnology Bulletin

基　　金：国家"863"项目(2007AA021403);浙江省自然科学基金资助项目(Y307469)

摘　　要：通过Websat对来源于NCBI公共数据库的2 447条石斛属(Dendrobium)核苷酸序列进行简单重复序列SSR的搜索,剔除冗余序列后,找到124个SSR位点。利用primer3.0软件设计引物75对,并通过改良的方法提取铁皮石斛DNA作为模板,对铁皮石斛(Dendrobium officinaleKimuraetMigo)的SSR引物进行筛选,选出21对有较清晰且稳定的目标扩增产物的引物,对8个种源的铁皮石斛进行多态性分析和聚类分析,得到8个种源的铁皮石斛进行遗传多样性和亲缘关系。Simple sequence repeats （ SSR ） of 2 447 nucleotide sequences of Dendrobium which were from NCBI, were investigated by using Websat online procedures. After eliminated redundancy, we got 124 SSR. 75 pairs of primers were designed by primer3.0 software and these primers were screened with the template of D. officinale. The results indicated that 21 pairs of them showed amplifications with polymorphisms. With polymorphism analysis and cluster analysis applied for 8 D. officinale species, we got their genetic diversities and paternities.

关 键 词：铁皮石斛 微卫星 SSR 多态性 

分 类 号：S567.239[农业科学—中草药栽培]