双氢青蒿素体外抑制人肝癌细胞株HepG2生长作用的实验研究  

The Experimental research of the effect of Dihydroartemisinin inhibition on the cells of HepG2

作　　者：王强[1] 武华[1] 

机构地区：[1]山西医科大学第一临床医学院普外科,太原030001

出　　处：《中国医疗前沿》2012年第11期9-10,共2页China Healthcare Innovation

摘　　要：目的研究双氢青蒿素(DHA)对体外培养的人肝癌细胞株HepG2的生长抑制作用及诱导凋亡作用。方法常规体外培养人肝癌细胞株HepG2,将不同浓度DHA在不同时间内作用于人肝癌细胞株HepG2,采用四甲基偶氮唑盐比色法(MTT)检测细胞生长抑制率,应用流式细胞仪检测细胞凋亡情况。结果不同浓度DHA均可抑制体外培养的人肝癌细胞株HepG2细胞生长,并可诱导其发生凋亡,且呈一定的剂量-效应、时间-效应关系。结论 DHA对体外培养的人肝癌细胞株HepG2有明显的生长抑制作用及诱导凋亡作用。Objective To study the effect of Dihydroartemisinin inhibition on the cells of Human hepatoma cell lines HepG2.Methods The cultured Human hepatoma cell lines HepG2 were treated with different concentration of DHA and different incubation time.MTT was used to detect the cell growth inhibition rate,and The fluorescence flow cytometry（FCM） DNA was used to study the cell apoptosis.Result Dihydroartemisinin significantly inhibits the growth of Human hepatoma cell lines HepG2,inducing apoptosis.And revealed some dose-time-effect relationship.Conclusion Dihydroartemisinin has obvious inhibition effect on Human hepatoma cell lines HepG2。

关 键 词：双氢青蒿素 肝癌细胞株HEPG2 抗肿瘤作用 

分 类 号：R392.5[医药卫生—免疫学]