金纳米材料复合型光敏剂介导的近红外光照治疗对于4T1乳腺癌细胞生长抑制效果的实验研究  

Growth inhibition of 4T1 breast cancer cells using Au nanoparticles enhanced photosensitizer and near infrared illumination

作　　者：刘晴[1] 谢欣[1] 张玥[1] 刘飞[1] 张宾[1] 白净[1] 

机构地区：[1]清华大学医学院生物医学工程系,北京100084

出　　处：《国际生物医学工程杂志》2012年第3期129-132,136,I0001,共6页International Journal of Biomedical Engineering

基　　金：国家973计划项目（2011CB707701）;国家重大科学仪器设备开发专项（2011YQ030114）;国家自然科学基金资助项目（81071191,60831003,30930092,30872633）;北京市自然科学基金项目（3111003）;清华-裕元基金

摘　　要：目的 对金纳米材料复合型光敏剂介导的近红外光照治疗效果进行评价.方法 将传统光敏剂吲哚菁绿（ICG）包裹在特定长径比的金纳米棒（AuNR）上,制备得到新型光敏剂——吲哚菁绿-金纳米棒（ICG-AuNR）,并使用该复合型光敏剂对小鼠乳腺癌细胞4T1进行近红外光照治疗实验研究.结果 复合型光敏剂的荧光强度为同浓度游离ICG溶液的4倍；同时,与相同浓度的游离ICG溶液相比,小鼠乳腺癌细胞4T1在复合型光敏剂介导的近红外光照治疗中表现出更低的细胞存活率.结论 与相同浓度的游离ICG溶液相比,金纳米材料复合型光敏剂对肿瘤细胞具有更有效的生长抑制以及治疗作用.Objective To evaluate the treatment efficiency of Au nanopartieles enhanced photosensitizer using near-infrared illumination. Methods Specific length-diameter-ratio Au Nanorods （AuNR） was coated with traditional photosensitizer indocyanine green （ICG）, producing the novel photosensitizer ICG-AuNR. In order to evaluate the treatment efficiency, in-vitro near-infrared phototherapy experiments were performed on 4T1 mouse breast cancer cells. Results The fluorescent intensity of ICG-AuNR is three times stronger than that of free ICG of the same concentration. Compared with free ICG of the same concentration, cell viability of 4T1 cells reduced in the near-infrared illumination treatment with the novel photosensitizer ICG-AuNR. Conclusion In comparison with free ICG of the same concentration, the novel photosensitizer ICG-AuNR proves to he more effective on growth inhibition of cancer cells using near-infrared illumination treatment.

关 键 词：金纳米棒 吲哚菁绿 4T1乳腺癌细胞 光照治疗 

分 类 号：TB383[一般工业技术—材料科学与工程] R979.1[医药卫生—药品]