胰岛素皮下给药和腹腔给药对KK小鼠自由基代谢的影响  

Effects of subcutaneous and intraperitoneal administration of insulin to KK mice＇s oxygen free radicals metaholism

作　　者：王欣然[1] 邓姗姗[1] 张超[1] 徐蓉[1] 唐丽娜[1] 魏洁[2] 孙洪范[1] 胡刚[2] 

机构地区：[1]中国医学科学院北京协和医学院生物医学工程研究所,天津市生物医学材料重点实验室,天津300192 [2]卫生部老年医学重点实验室,卫生部北京老年医学研究所,卫生部北京医院,北京100730

出　　处：《国际生物医学工程杂志》2012年第3期155-159,共5页International Journal of Biomedical Engineering

基　　金：天津市应用基础重点项目（08JCZDJC16000）

摘　　要：目的 观察皮下与腹腔2种胰岛素给药方式对2型糖尿病模型氧自由基水平的影响.方法选用C57BL/6J小鼠作为正常对照组（C组,n=9）.KK小鼠随机分为腹腔注射胰岛素组（i.p.组,n=9）、皮下注射胰岛素组（s.c.组,n=9）、未治疗组（U组,n=9）.i.p组与s.c.组每天给予一定量胰岛素（胰岛索注射液与精蛋白锌胰岛素注射液按体积比2：1混合）治疗,使血糖维持在正常水平（6±1.5） mmol/L,连续给药1个月.分别检测每组的血清、肝脏、肾脏、心脏超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）活性和丙二醛（MDA）含量.结果U组的肝、肾、心和血清中SOD及GSH-PX活性显著降低,MDA含量显著升高.2种给药方式皆可使血清SOD及GSH-PX活性提高、MDA含量下降至正常对照组水平,但腹腔给药方式的作用更强.2种给药方式可使肝脏MDA含量显著下降,且作用无显著差异；但皮下给药组提高肝脏SOD活性的作用优于腹腔给药组,腹腔给药组提高肝脏GSH-PX活性的作用优于皮下给药组.腹腔给药方式降低肾脏MDA含量、提高SOD活性的作用显著优于皮下给药组.2种给药方式降低心脏组织MDA的效果相同.结论2种给药方式都可以使KK小鼠的血清、心、肝、肾MDA水平降至正常对照组水平,但2种给药方式在各脏器提高抗氧化能力的模式有所不同.腹腔给药方式降低血清、肾脏、MDA含量的效果更好.Objective To observe the subcutaneous and intraperitoneal insulin injection＇s effect of the level of oxygen free radicals of type 2 diabetes model. Methods C57BL/6J mice were chosen as normal control group （C group, n=9）. KK mice were randomly divided into intraperitoneal injection of insulin group （i.p. group, n=9）, the subcutaneous insulin group （s.c. group, n=9） and untreated group （U group, n = 9）. The i.p. group and the s.c. groups were given certain amount of insulin （insulin injecta and protamine insulin injecta by volume ratio of 2：1 mixture） for one month, maintained the GLU at normal levels （6±1.5） mmol/L. SOD, GSH-PX activity and MDA content of serum, liver, kidney and heart in each group were detected. Results The liver, kidney, heart and serum＇s SOD and GSH-PX activity significantly reduced and MDA content significantly increased in the U group. Both kinds of delivery methods could increase serum SOD and GSH-PX activity and reduce the content of MDA to the normal control group level, but the intraperitoneal injection had stronger effect. Two kinds of delivery methods could both reduce the MDA content of liver, and had almost the same effect; but the subcutaneous injection group had better effect on increasing the liver＇s SOD activity, and the intraperitoneal injection had better effect on increasing liver＇s GSH-PX activity. Intraperitoneal injection had better effect on reducing kidney＇ s MDA content and increased SOD activity. Two kinds of delivery methods had the same effect on reducing the heart＇s MDA content. Conclusion The two delivery methods can both make the MDA levels of KK mice in serum, heart, liver and kidney fall to as normal as that of control group, but the two delivery methods have different ways of improving the antioxidant capacity in different organs. Intrapefitoneal injection can reduce MDA content in serum and kidney better.

关 键 词：糖尿病 胰岛素 氧自由基 

分 类 号：R285.5[医药卫生—中药学]