不同细胞培养皿对激光共聚焦显微镜成像效果的影响  

Effects of different types of cell culture dishes on fluorescent image with confocal microscope

作　　者：庞丽云[1] 王海[1] 刘焕来[1] 徐毓琪[1] 朱敦皖[1] 

机构地区：[1]中国医学科学院北京协和医学院生物医学工程研究所,天津300192

出　　处：《国际生物医学工程杂志》2012年第3期177-180,I0005,I0006,共6页International Journal of Biomedical Engineering

摘　　要：目的 探讨3种不同玻片厚度的激光共聚焦专用细胞培养皿制备的细胞样品在激光共聚焦显微镜（CLSM）下采集的荧光染色图像差异.方法通过活细胞及固定后细胞荧光染色实验,采用激光共聚焦显微镜观察细胞形态及荧光亮度,检测不同条件下成像的平均荧光强度,考察3种厚度玻片（0.085～0.13、0.13～0.16、0.16～0.19mm）的激光共聚焦专用细胞培养皿之间显微成像的差异.结果在观察活细胞线粒体和固定细胞骨架时,玻片厚度为0.13～0.16 mm的皿荧光成像清晰效果均表现为最好,玻片厚度为0.16～0.19mm的皿次之,玻片厚度为0.085～0.13 mm的皿较差.结论玻片厚度对激光共聚焦显微镜成像效果有明显的影响,玻片厚度为0.13～0.16 mm的培养皿其细胞形态舒展,染色后细胞骨架清晰,荧光强度最强,成像效果最佳.Objective To investigate the effects of different thickness of cell culture dishes on fluorescent image with confocal microscope. Methods The fluorescent staining experiments of live cells and fixed cells were used to determine the differences among three dishes with different thickness coverslips of 0.085- 0.13 mm, 0.13-0.16 mm and 0.16-0.19 ram, while the cell appearance, fluorescence lightness and mean of fluorescence intensity were studied with confocal microscope. Results Demonstrated by the results of cytoskeleton staining experiments, the dish with 0.13-0.16 mm thickness coverslip was the best choice for confocal microscope, the dish with 0.16-0.19 mm thickness coverslip was the second one, the dish with 0.16-0.19 mm thickness coverslip was the last one. Conclusion The dish with 0.13-0.16 mm thickness coverslip is the best choice for confocal microscope. On this type of dish, the cytoskeleton is unfolding and clear after staining. The intensity of fluorescence is the strongest, and the imaging effect is the best.

关 键 词：细胞培养皿 激光共聚焦显微镜 荧光染色 

分 类 号：R318.51[医药卫生—生物医学工程]