沙美特罗对哮喘模型大鼠气道炎症的作用及其机制  

Effect of salmeterol on airway inflammation of rats with bronchial asthma and its mechanism

作　　者：袁晓梅[1,2] 孙浩杰[3] 王静[2] 

机构地区：[1]郑州大学第一附属医院呼吸科,河南郑州450052 [2]新乡医学院一附院呼吸内科,河南卫辉453100 [3]新乡医学院一附院核医学科,河南卫辉453100

出　　处：《吉林大学学报（医学版）》2012年第4期717-720,822,共4页Journal of Jilin University：Medicine Edition

基　　金：河南省卫生厅科研基金资助课题(200804056)

摘　　要：目的:观察沙美特罗(SLM)对哮喘模型大鼠支气管肺泡灌洗液(BALF)中炎性因子的影响,探讨其在哮喘治疗中的作用机制。方法:选取SD大鼠48只随机分为空白对照组、模型组、SLM组及普萘洛尔加SLM组。收集各组大鼠BALF,光镜下计数嗜酸性粒细胞(EOS),原位末端标记法(TUNEL)检测EOS凋亡指数,酶联免疫吸附试验(ELISA)法测定白细胞介素5(IL-5)和嗜酸粒细胞趋化因子(Eotaxin)水平。HE染色观察肺组织病理改变。结果:模型组和普萘洛尔加SLM组大鼠BALF中EOS计数、Eotaxin和IL-5水平均显著高于空白对照组(P<0.01),EOS凋亡指数显著低于空白对照组(P<0.01)。SLM组大鼠BALF中EOS计数、IL-5和Eotaxin水平显著低于模型组(P<0.01),EOS凋亡指数显著高于模型组(P<0.01)。模型组和普萘洛尔加SLM组大鼠肺组织呈明显炎症变化,SLM组大鼠肺组织呈轻度炎症变化。结论:SLM可能通过β2肾上腺素受体降低哮喘大鼠BALF中IL-5和Eotaxin水平从而降低EOS的数量来发挥抗炎作用。Objective To observe the effect of salmeterol（SLM） on inflammatory factors in bronchoalveolar lavage fluid（BALF） of rats with bronchial asthma,and to investigate the therapeutic mechanism of salmeterol on bronchial asthma.Methods 48 SD rats were divided into 4 groups randomly：blank control group,model group,SLM group and inderal ＋SLM group.BALF of rats in various groups was collected.Then the eosinophil（EOS） was counted under light microscope,the apoptotic index of EOS was detected by TUNEL,and the levels of interleukin-5（IL-5） and Eotaxin were detected by ELISA.The pathological changes of lung tissues were observed by HE staining.Results The levels of EOS,Eotaxin and IL-5 in BALF of rats in model group and inderal ＋SLM group were all higher than those in blank control group（P〈0.01）.The apoptotic indexes in model group and inderal ＋SLM group were lower than that in blank control group（P〈0.01）.The levels of EOS,Eotaxin and IL-5 in BALF of rats in SLM group were all lower than those in model group（P〈0.01）.The apoptotic index in SLM group was higher than that in model group（P〈0.01）.Inflammation was significant in lung tissues in model group and inderal ＋SLM group.Inflammation was mild in lung tissues in SLM group.Conclusion The therapeutic mechanism of SLM on bronchial asthma may be related to lowering the number of EOS by the way of adrenoceptor-β2 to depress Eotaxin and IL-5 in BALF of asthma rats.

关 键 词：沙美特罗 哮喘 嗜酸性粒细胞 白细胞介素5 嗜酸粒细胞趋化因子 细胞凋亡指数 大鼠 

分 类 号：R562.25[医药卫生—呼吸系统]