高效液相色谱法测定保元清血颗粒中黄芪甲苷含量方法的建立及评价  

Establishment and evaluation of determination of astragaloside Ⅳ in Baoyuanqingxue granules by HPLC

作　　者：孙聪[1] 韩业超[2] 吴强[3] 

机构地区：[1]长春中医药大学基础医学院生物化学与分子生物学教研室,吉林长春130117 [2]吉林省兽药饲料监察所,吉林长春130062 [3]吉林大学中日联谊医院中医科,吉林长春130033

出　　处：《吉林大学学报（医学版）》2012年第4期805-808,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅社会发展重点项目资助课题(20110950);吉林省中医药管理局科技项目资助课题(2010076)

摘　　要：目的:探讨影响保元清血颗粒中黄芪甲苷含量测定的各种因素,分析黄芪甲苷含量测定的提取方法及色谱条件,建立黄芪甲苷含量测定的有效方法。方法:保元清血颗粒中黄芪甲苷含量测定前处理采用甲醇超声提取法,检测采用高效液相色谱(HPLC)法,色谱柱为SB-C18柱(Agilent公司生产,ZORBAX,4.6mm×150mm,5μm),流动相为乙腈-水(36∶64),流速为1.00mL.min-1,柱温为30℃,进样量为20μL,UV检测器检测波长为203nm。结果:在本色谱条件下,黄芪甲苷与其他峰分离良好,在0.010 03～0.200 60g.L-1范围内呈良好的线性关系(r=0.999 9);平均加样加收率为98.4%,RSD为0.8%(n=6)。结论:采用高效液相色谱法-紫外检测器(HPLC-UV)测定保元清血颗粒中黄芪甲苷含量,方法便捷、准确,可作为黄芪甲苷含量测定的质量控制方法。Objective To investigate the health condition of the old people in the urban and rural communities of Changchun city,and to analyze its influencing factors and provide evidence for elderly to prevent related diseases and the government to make care strategies.Methods ADL scale,SF-36 scale and self-designed questionnaire were used to investigate 600 old people from Guilin Road community in urban,Jingyue Development community in the urban and rural junction and Jiangjia community in rural by grading and systematic sampling.Some physical examinations were performed at the same time.SPSS 13.0 software was adopt to deal with the data and compare the conditions of the three communities.Results The morbidity of chronic diseases in old people was 77.14%,and they were 87.36%,83.61% and 71.22% in urban,urban and rural junction and rural communities.The morbidities of various chronic diseases were as follow：heart disease 51.43%,hypertension 36.96%,arthritis 34.11%,cerebrovascular diseases 28.04%,respiratory diseases 23.04%,cataract 8.04%,malignant tumors 3.92%,Parkinson’s disease 1.42%,disability 0.53%.The old people who had at least two kinds of chronic diseases occupied 67.30%.The downtown community had higer morbidities of hypertension,heart disease,cerebrovascular diseases and arthritis than the other two communities（P〈0.001）.Conclusion The old people of Changchun communities has higher morbidity of chronic diseases.The medical workers in community health service center should provide more health guidance to improve the old people’s health.

关 键 词：保元清血颗粒 黄芪甲苷 高效液相色谱法-紫外检测器 

分 类 号：Q503[生物学—生物化学]