响应面法优化东北接骨木总黄酮的超声波提取工艺  

Optimization of Total Flavonoids Extraction fromSambucus mandchurica Kitag by Response Surface Method

作　　者：张爽[1] 刘淑英[1] 于莉莉[1] 仲文晶[1] 刘洪章[1] 

机构地区：[1]吉林农业大学生命科学学院,吉林长春134001

出　　处：《北方园艺》2012年第14期11-14,共4页Northern Horticulture

基　　金：吉林省科技厅资助项目(20100254)

摘　　要：对乙醇体积分数、超声时间、液料比、超声温度进行单因素试验,在此基础上选取乙醇体积分数、超声时间、液料比进行响应面法优化东北接骨木总黄酮的超声波提取工艺的研究,利用响应面分析这3个因素对东北接骨木总黄酮提取的影响。结果表明:东北接骨木中总黄酮超声提取的最佳工艺为:乙醇体积分数为76.03%,超声时间48.86min,液料比为19.53mL/g,黄酮提取量达到5.79692mg/g。Through four factors that is ethanol concentration,extraction time, liquid-solid ratio and extraction temperature for the single factor experiments,on the basis of selecting thethanol concentration,extraction time, liquid-solid ratio for the optimization of total flavonoids extraction from Sambucus rnandchurica Kitag,the three factors for extraction yield of the total flavonoids by RSM were studied. The results showed that best condition to ultra the total flavonoids from Sambucus mandchurica Kitag were ethanol concentration 76. 03%, ultrasound time 48. 86 rain and liquid solid ratio 19. 53 mL/g,the extraction yield of the total flavonoids was 5. 79692 mg/g.

关 键 词：东北接骨木 超声提取 总黄酮 响应面法 

分 类 号：S567.19[农业科学—中草药栽培]