全氟辛烷磺酸与蛋白质体系的共振光散射光谱及其分析应用  

Resonance Light Scattering Spectra of Perfluorooctane Sulfonate-Protein System and Its Analytical Application

作　　者：吴飞[1] 朱进[1] 谭克俊[1] 

机构地区：[1]三峡库区生态环境教育部重点实验室,西南大学化学化工学院,重庆400715

出　　处：《应用化学》2012年第8期969-973,共5页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(20877063);西南大学大型仪器设备开放基金资助项目

摘　　要：研究了牛血清白蛋白(BSA)与全氟辛烷磺酸(PFOS)相互作用的共振光散射(RLS)光谱,建立了PFOS的共振光散射分析方法。在pH值为4.1的BR缓冲溶液中,全氟辛烷磺酸根阴离子与质子化的BSA通过静电引力和疏水作用形成离子缔合物,引起共振光散射强度(IRLS)显著增强,最大散射波长位于285.0 nm处,增强的散射信号强度与PFOS浓度在0.2～25.0μmol/L范围内呈线性关系,据此建立了测定PFOS的光散射分析方法,检出限为20.0 nmol/L。讨论了体系的最佳反应条件及外来物质的干扰,并探讨了反应机理。建立的共振光散射法用于环境水样中PFOS的测定,RSD≤4.4%。The interaction of bovine serum albumin （BSA） with perfluorooctane sulfonate （PFOS） was investigated with resonance light scattering（RLS） spectroscopy. A RLS method for the determination of PFOS was established. In pH = 4. 1 Britton-Robinson buffer solution, perfluorooctane sulfonate anions reacted with the protonated BSA by electrostatic forces and hydrophobic interactions to form ion-association complexes and resulted in greatly enhanced resonance light scattering signals characterized by a peak at 285.0 nm. The RLS intensity of system was proportional to the content of PFOS in the range of 0. 2 - 25.0 μmol/L, and the limit of detection was 20. 0 nmol/L. The optimum reaction conditions and interference of foreign substances were investigated. This RLS method was applied to the determination of PFOS in environmental samples with RSD ≤4. 4%.

关 键 词：牛血清白蛋白 全氟辛烷磺酸 共振光散射 

分 类 号：O657.3[理学—分析化学]