无液氮冷冻小鼠精子的研究  

Research on Preservation Mouse Spermatozoa without Liquid Nitrogen

作　　者：巢时斌[1,2] 李建春[1] 罗世明[2] 高国兰 

机构地区：[1]南昌大学第一附属医院生殖医学中心,南昌330006 [2]中国科学院动物研究所,生殖生物学国家重点实验室,北京100101 [3]航空总医院妇产科,北京100012

出　　处：《西北农业学报》2012年第6期1-5,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家基础研究项目(2011CB944501);江西省教育厅青年基金(GJJ10076)

摘　　要：目前实验室通常用液氮加冷冻保护剂法保存小鼠精子,这种方法操作复杂而且会带来一定的安全隐患。通过对受精能力、胚胎发育潜能和胚胎组蛋白H3第4位赖氨酸残基位点上三甲基化(H3K4-TriM)的比较,探讨-20℃不加冷冻保护剂冷冻小鼠精子的效果。结果表明,-20℃不加冷冻保护剂冷冻的精子能通过单精子卵胞浆内注射重建受精能力,保持较好的胚胎发育潜能,不改变其早期胚胎H3K4-TriM的组蛋白修饰模式。可见,-20℃保存小鼠精子的方法简单、有效,有一定的应用价值。The most accepted protocol of sperm preservation is freezing in liquid nitrogen （LN2） with cryoprotectants. However, this method requires constant supplementation of the LN2 and also involves some safety issues in transporting. Here, we introduced a method without cryoprotectants and preserving mouse spermatozoa with ordinary refrigerator. The simple method could maintain fertiliza- tion ability of freezing spermatozoa and support embryo preimplantation development via ICSI. Moreover, we detected dynamic histone H3K4 trimethylation in the embryos derived from the freezing spermatozoa. No difference was found between the fresh sperm and freezing sperm. In conclusion, the method is a simple, effective, and safety spermatozoa preservation.

关 键 词：精子冷冻 单精子卵胞浆内注射 表观遗传学 组蛋白甲基化 

分 类 号：S865.13[农业科学—野生动物驯养]