梭梭EF-hand CaBP基因克隆及序列分析  

Cloning and Sequence Analysis EF-hand CaBP Gene from Haloxylon ammodendron

作　　者：周晓燕[1,2] 甘晓燕[1] 石磊[1] 陈虞超[1] 李苗[1] 宋玉霞[1] 

机构地区：[1]宁夏农业生物技术重点实验室,银川750002 [2]宁夏大学生命科学学院,银川750021

出　　处：《西北农业学报》2012年第6期47-52,57,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：宁夏回族自治区科技攻关宁陕合作项目资助(KGZ-16-07-02)

摘　　要：采用RT-PCR、RACE等方法从超旱生、耐盐植物梭梭(Haloxylon ammodendron)中扩增出EF-handCaBP(EF-hand calcium-binding protein)基因的cDNA序列(GenBank登录号为JQ023165),其开放阅读框为732bp,推测的氨基酸序列全长为243个氨基酸残基。运用SMART、GOR4、TMPred等生物信息学软件对梭梭EF-hand CaBP功能域、跨膜结构进行分析显示,梭梭EF-hand CaBP含有26个氨基酸的信号肽序列及4个保守的钙结合位点(EF-hand),并且存在2种跨膜模型,推测梭梭EF-hand CaBP基因可能定位于液泡膜或叶绿体膜上。Through RT-PCR（reverse transcription polymerase chain reaction）and RACE（rapid amplifi- cation of cDNA ends）, a 723bp fragment containing entire EF-hand CaBP gene coding region of 243 amino aeids（aa） has been obtained from the extreme xerophyte and salt-tolerant plant Haloxylon am- modendron （GenBank accession No. JQ023165）. Analysis of the EF-hand CaBP functional domains and transmembrane model with SMART, GOR4 and TMPred bioinformatics software shows that Haloxylon ammodendron EF-hand CaBP contains 26 amino acid signal peptide and four conserved calcium binding sites（EF-hand）, and exists two transmembrane model, specu be located in the vacuolar membrane or chloroplast membrane.

关 键 词：梭梭 钙结合蛋白 EF-hand手型 序列分析 

分 类 号：S793.9[农业科学—林木遗传育种]