甘蓝小孢子培养中花蕾长度与细胞单核期的关系  

Relationship between the Length of Buds and Uninucleate Stage of Isolated Microspore Culture in Cabbage

作　　者：张恩慧[1] 马英夏[1] 杨安平[2] 许忠民[1] 程永安[1] 马勇斌[1] 

机构地区：[1]西北农林科技大学园艺学院,农业部西北地区园艺作物生物学与种质创制重点实验室,陕西杨凌712100 [2]杨凌职业技术学院生物工程系,陕西杨凌712100

出　　处：《西北农业学报》2012年第6期124-128,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家大宗蔬菜产业技术体系项目(CARS-25);“十一·五”国家科技支撑计划资助项目(2006BAD01A7-2-04);陕西省13115重大科技专项(ZDKG-126);西安市农业科技攻关资助项目(GG06090)

摘　　要：以甘蓝杂种一代品种抽薹植株初花期的花蕾为供体材料,通过镜检观察得出,在甘蓝植株1次分枝花序上花蕾肉眼可见间隔为5d时,花粉小孢子形状呈现在不明显的三裂形与正圆形间,细胞发育正处在单核靠边时期;花蕾生长的长度为4.50～5.49mm,花粉细胞发育所处的单核靠边期的小孢子平均所占比例为76%。采用NLN-14培养基对不同发育时期的小孢子诱导培养,可见所选花蕾的小孢子多数发育处在单核靠边期,小孢子离体培养容易诱导出胚状体。The buds of F1 cabbage were selected as the research materials in this study, microspores were observed under inverted microscope. The buds from the first branch of cabbage to be examined by the unaided eye, the results showed that microspore from three splits form to perfect circular form after five days, the development of microspore was at late uninucleate stage, the length of buds was in the range of 4.50 to 5.49 mm, the rate of microspore at late uninucleate stage was 76%. Different development stage microspore were cultured by NLN-14, it was easy to induce embryoids when the majority microspore development stage was late uninucleate stage.

关 键 词：甘蓝 花蕾 小孢子 发育 胚状体 

分 类 号：S635.1[农业科学—蔬菜学]