川楝树皮中川楝素质量分数的液相色谱检测  

Determination of Toosendanin in the Bark of Melia Toosendan Sieb. et Zuccc. by High Performance Liquid Chromatography

作　　者：高保卫[1] 张璟[1] 冯俊涛[1] 王智辉[1] 张兴[1] 

机构地区：[1]西北农林科技大学无公害农药研究服务中心,陕西省生物农药工程技术研究中心,陕西杨凌712100

出　　处：《西北农业学报》2012年第6期178-181,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(2010ZDKG-73);西北农林科技大学大型仪器新功能开发项目(dysb0901)

摘　　要：建立液相色谱仪快速检测川楝树皮中川楝素质量分数的方法。最佳提取条件为甲醇体积分数70%、回流温度70℃、回流时间1h;色谱条件为甲醇与水的混合物[V(甲醇)∶V(水)=65∶35)]作流动相,检测波长215nm,流速1.0mL/min,柱温25℃,进样量5μL;川楝素得率2.81‰,添加水平为0.75～3.00mg时,回收率为97.33%～99.33%。该方法能准确快速检测样品川楝素的质量分数。A reversed-phase high performance liquid chromatographic method was developed for determining toosendanin in the bark of Melia toosendan Sieb. et zuccc. The optimum extraction condition for toosendanin was as following： Volume fraction of methanol 70%, extraction temperature 70℃, extraction time 1 h. The method for HPLC assay was with methanol-water IV（methanol） ＂ V（water） =65 ＂ 35] as mobile phase and ultraviolet detector at 215 nm. The column temperature was 25 ℃ and the flow rate was 1 mL/ min, the injection volume was 5ul. The yield of toosendanin is 2.81‰. The recoveries at spiked concentrations of 0.75-3.00 mg ranged from 97.33% to 99.33%. It is showed this method is simple, accurate and can be used for determining toosendanin in the bark of Melia Toosendan Sieb. et Zucc.

关 键 词：回流提取 液相色谱 川楝素 

分 类 号：R284[医药卫生—中药学]