In vitro Propagation of Walnut （Juglans regia） by Nodal Explants  

作　　者：Layla Shaaban Mohammed A1-Mizory Azad Ahmed Mayi 

机构地区：[1]Department of Horticulture, School of Plant Production, Faculty of Agriculture and Forestry, University of Duhok, Kurdistan Region,lraq

出　　处：《Journal of Agricultural Science and Technology(B)》2012年第6期665-670,共6页农业科学与技术（B）

摘　　要：Micropropagation techniques were set up for walnut. Many plantlets were obtained by culturing node explants in MS medium,with 30 g/L sucrose, 7 g/L agar, and different concentrations of BA or Kin alone and different concentrations of BA ＋ NAA or BA ＋ Kin. BA at concentration （2 mg L-1 BA） gave the best results in percentage response and number of shoots/plant （100%, 2.84） respectively. While, Kin at 3 mg L-1 gave the highest average shoot length （2.81 cm）. However, MS medium supplemented with 2.5 mg L-1 ＋ 0.5 mg L-1 Kin gave the best results compared with all treatment shoot/explants and the highest number of leaves/explants （3.7, 8.2） respectively. While the highest length of shoot （4.7 cm） in MS medium supplemented with 3 mg L-1 Kin. and a high rooting percentage up to 60% at MS medium supplemented with 1 mg L-1 IBA （Indole-3-butyric acid） was obtained.

关 键 词：MICROPROPAGATION WALNUT single-node explants cytokinins. 

分 类 号：S682.11[农业科学—观赏园艺] S665.1[农业科学—园艺学]