Cell Viability of Byrsonima intermedia A Juss Calli  

作　　者：Luciano Coutinho Silva Renato Paiva Daiane Peixoto Vargas Diogo Pedrosa Correa da Silva Rairys Cravo Herrera Sandro Barbosa Antonio Paulino da Costa Netto 

机构地区：[1]Biology Department, Plant Physiology Sector, Federal University of Lavras (UFLA), Caixa Postal 3037, CEP 37200-000, Lavras,Minas Gerais, Brasil [2]Embrapa Clima Temperado, Rodovia BR 392, km 78 CP 403, Pelotas, Rio Grande do Sul, CEP: 96010-971, Brasil [3]Universidade Federal do Pare (UFPA), Campus Universitfrio de Altamira, Rua Coronel Jose Porfirio, 2515, Sao Sebastiao, CEP68372-040, Altamira, Pard, Brasil [4]Departamento de Ciencias Biologicas e da Terra, Universidade Federal de Alfenas (UNIFAL), MG, Rua Gabriel Monteiro da Silva, 700, Centro, CEP 37130-000, Alfenas, Minas Gerais, Brasil [5]Laboratorio de Fisiologia Vegetal, Universidade Federal de Goifs (UFG), Rua Riaehuelo, S/N, Unidade Jatoba Setor SamuelGraham, 75804-020, Jatai, GO, Brasil

出　　处：《Journal of Agricultural Science and Technology(B)》2012年第6期713-720,共8页农业科学与技术（B）

摘　　要：Byrsonima intermedia A Juss. is a species with pharmacological properties from the Brazilian Cerrado that shows difficulties related to sexual propagation. Research on cell viability may provide useful information for the selection of cells with embryogenic potential during the callus culture, Within this context, our research is aimed at establishing the cell viability of calli from Byrsonima intermedia leaf segments. The calli went through three subculture phases, of 60 days each, in MS medium with 0.09 M sucrose, 0.6% agar, pH 5.8 and 4.52 laM 2,4-D. The calli were stored in dark conditions and samples were collected every 10 days from each subculture for viability tests with fluorescein 3,6-diacetate （FDA） and 2,3,5-triphenyltetrazolium chloride （TTC）. The staining methods allowed quantifying cell viability in each subculture. The best results from the FDA tests were obtained at 21, 25 and 29 days for the first, second and third subcultures respectively, with 53,86%, 61.88% and 53.73% viable cells. Regarding the TTC test, the largest absorbance values were obtained at 21, 27 and 28 days for the first, second and third subcultures respectively. Fluorescence and spectrophotometry analyses were efficient for determination of cell viability during callus cultivation period.

关 键 词：Cell viability fluorescein 3 6-diacetate 2 3 5-tripheniltetrazolium chloride SUBCULTURE tissue culture native plant. 

分 类 号：S531[农业科学—作物学] Q943.1[生物学—植物学]