陕西及宁夏盐渍土中拮抗放线菌资源研究  

Study on the Antagonistic Actinomycetes Resources in Saline Soil of Shannxi and Ningxia

作　　者：王海利[1] 来航线[2] 马玥[2] 费关键 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]西北农林科技大学资源环境学院,陕西杨凌712100 [3]兴平市植保植检站,陕西兴平713100

出　　处：《西北农业学报》2012年第5期54-59,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金："十二五"国家科技支撑计划项目(2012BAD14B11)

摘　　要：以分离自盐渍土中的放线菌作为供试菌株,以养殖业中常见的9种动物病原菌作为靶标菌进行拮抗放线菌研究,并对4株优良拮抗菌株进行初步鉴定。结果表明,供试的1 172株放线菌主要以链霉菌属为主,占到88.82%,有拮抗活性的菌株551株,占到47.01%;供试放线菌对不同靶标菌的抑菌作用差异较大,对金黄色球菌和无乳链球菌拮抗效果较好;菌株01F10为白孢类群,与Streptomyces qinglanensis亲缘关系最近,序列相似度为99.11%;菌株02H13和14H03为粉红孢类群,与Streptomyces gobitricini序列相似度达到100%;菌株07F10为灰褐类群,与Streptomyces sparsus亲缘关系最近,序列相似度为99.69%。Actinomyces stranins were isolated from saline soil and 9 bacteria strains were used as the indicator in this test. 4 antagonistic strains with good antagonistic effect were identified. The results showed： the main sort of the 1172 actinomycetes in these areas is streptomyces, the rate was 88.82 %. And 551 strains showed antagonistic effects. Actinomycetes tested for different target bacte- ria have different effects, and have better antagonistic effects on Staphyloccocus aureus and bovine Streptococcus agalactiae. 4 excellent antagonistic actinomucetes identification results showed that the strain 01F10 named Albosporus, close related to Streptomyces qinglanensis, the sequence similarity is 99.11% ;The strain 02H13 and 14H03 named Roseosporus, close related to Streptomyces gobitricini, the sequence similarity are 100% ;The strain 07F10 named Griseofuscu, close related to Streptomyces sparsus qinglanensis, the sequence similarity is 99.69%.

关 键 词：盐渍土 动物病原菌 拮抗性放线菌 鉴定 

分 类 号：S154.3[农业科学—土壤学] S852.6[农业科学—农业基础科学]