斑蝥酸钠联合多西紫杉醇诱导A_(549)细胞凋亡及对Caspase-8表达的影响  

Research on the Combined Effect of Sodium Cantharidinate and Docetaxel on Adenocarcinoma Cell Apoptosis and the Activities of Caspase-8

作　　者：许惠溢[1] 奚燕[2] 钟以胜[3] 曾寰[1] 林碧英[1] 

机构地区：[1]厦门市第二医院,福建厦门361021 [2]上海中医药大学附属龙华医院,上海200126 [3]厦门市中医院,福建厦门361009

出　　处：《中国现代应用药学》2012年第7期586-590,共5页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的探讨斑蝥酸钠联合多西紫杉醇抑制人肺腺癌A549细胞增殖、诱导细胞凋亡的作用,初步探讨其作用机制。方法采用MTT法测定细胞增殖抑制率,Annexin V-FITC/PI染色,流式细胞仪测定细胞凋亡率;Western印迹法测定Caspase-8活性。结果斑蝥酸钠和多西紫杉醇均能抑制人肺腺癌A549细胞的增殖(P<0.05),合用与单用相比抑制率明显提高,诱导12 h时CI值为0.88,24 h时为0.96,36 h时为1.02。Annexin V-FITC/PI染色流式细胞仪检测提示斑蝥酸钠可增强多西紫杉醇的凋亡诱导作用。Western印迹法显示多西紫杉醇、斑蝥酸钠及联合加药组均可见凋亡相关蛋白Caspase-8的裂解条带,联合加药组的裂解条带最明显。结论多西紫杉醇和斑蝥酸钠均可明显抑制人肺腺A549细胞增殖诱导细胞凋亡,联合使用作用更强,多西紫杉醇和斑蝥酸钠诱导细胞凋亡可能与促进Caspases-8表达有关。OBJECTIVE To investigate the inhibition effects of combination of Docetaxel and Sodium Cantharidinate on human pulmonary adenocarcinoma. METHODS Adenocarcinoma cell line A549 was chosen and growth inhibition was measured both with MTT colorimetry and FCM assay. Caspase8 was detected by Western blot. RESULTS Either Docetaxel orSodium Cantharidinate can inhibit the proliferation of A549 cell respectively, however, Sodium Cantharidinate can increase the sensitivity 0fA549 cell to Docetaxel when using them at the same time. The difference between single use and combine two drugs is distinct（P〈0.05）. High-level Caspase8 expression was detected with Docetaxel or Sodium Cantharidinate in A549 cells by Western blot. CONCLUSION Sodium Cantharidinate can sensitize A549 cell to Docetaxel＇s effects in vitro. The induction of apoptosis may be related to expression of Caspase8.

关 键 词：斑蝥酸钠 多西紫杉醇 人肺腺癌A549细胞 细胞凋亡 CASPASE8 

分 类 号：R965.1[医药卫生—药理学]