海洋放线菌杀虫抗生素的一种快速筛选模型  

A Rapid Screening Assay for Insecticidal Antibiotic Produced by Marine Actinomycetes

作　　者：胡志钰[1] 刘三震[1] 黄浩 黄耀坚[1] 苏文金[1] 

机构地区：[1]厦门大学生物学系,厦门361005 [2]厦门海洋实业集团股份有限公司,厦门361005

出　　处：《海洋通报》2000年第4期36-41,共6页Marine Science Bulletin

摘　　要：报道了以卤虫为指示动物，采用双层琼脂扩散方法构建的一种杀虫微生物筛选模型。该模型具有灵敏度高，快速简便等优点，适合于海洋微生物杀虫活性物质的早期大量初筛工作。利用该模型对１２４０株分离于厦门海区潮间带的放线菌进行了初筛，杀虫阳性率为２．３％，展示了海洋微生物是杀虫活性物质研究和开发的重要来源。A rapid screening assay for insecticidal microorganisms is described in this paper. The double-layer agar and brine shrimp (Artemia salina ) used as bioassay, are involved. The assay is highly sensitive, and easy to operate, and is suitable for the early screening for insecticidal activity materials from marine microorganisms. 1240 cultures, isolated in the tidal zone of the Xiamen sea area, have been examined, and 2.3% is given as producing inseticidal activity materials. The present work indicates that marine microorganisms could be an important source of inseticidal activity substances.

关 键 词：海洋微生物 杀虫抗生素 筛选模型 

分 类 号：S482.39[农业科学—农药学]