Visualizing NO in live cells by confocal laser scanning microscopy  

作　　者：黄义梅 杨洪钦 沈秀球 王瑜华 郑莉琴 李晖 谢树森 

机构地区：[1]Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education,Fhjian Provincial Key Laboratory of Photonic Technology, Institute of Laser and Optoelectronics Technology, Fujian Normal University, Fuzhou 350007, China

出　　处：《Chinese Optics Letters》2012年第B06期161-163,共3页中国光学快报（英文版）

摘　　要：Determination of NO concentration in live cells is essential to evaluate its related cellular functions. In this letter, the concentration of NO in HeLa cells and rat dorsal root ganglion （DRG） neurons are studied by confocal laser scanning microscopy using DAF-2 DA as a fluorescence probe. The results show the fluorescence intensity of NO in HeLa cells is higher than that in DRG neurons, which indicats that the former exhibits higher NO concentration. Furthermore, the experimental conditions for low photobleaching and Dhototoxicitv are optimized.Determination of NO concentration in live cells is essential to evaluate its related cellular functions. In this letter, the concentration of NO in HeLa cells and rat dorsal root ganglion （DRG） neurons are studied by confocal laser scanning microscopy using DAF-2 DA as a fluorescence probe. The results show the fluorescence intensity of NO in HeLa cells is higher than that in DRG neurons, which indicats that the former exhibits higher NO concentration. Furthermore, the experimental conditions for low photobleaching and Dhototoxicitv are optimized.

分 类 号：Q2[生物学—细胞生物学] S633.401[农业科学—蔬菜学]