羟基红花黄色素A缓解脂多糖诱导内皮细胞炎症因子表达升高的研究  

Study of hydroxysafflow yellow A to attenuate acute lung injury model of endothelial cell inflammatory factor expression induced by LPS

作　　者：朱煜[1] 宋丽娟[2,3] 臧宝霞[2,3] 边宝林[4] 金鸣[2,3] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]首都医科大学附属安贞医院 [3]北京市心肺血管疾病研究所药理研究室 [4]中国中医科学院中药研究所化学室

出　　处：《心肺血管病杂志》2012年第4期484-487,共4页Journal of Cardiovascular and Pulmonary Diseases

基　　金：北京市自然科学基金资助项目(编号:7102025)

摘　　要：目的:研究羟基红花黄色素A(HSYA)抑制脂多糖(LPS)诱导的脐静脉血管内皮细胞(Eahy926细胞)炎症因子表达升高的作用。方法:采用RT-qPCR法测定Toll样受体4(TLR4)、白介素6(IL-6)、白介素1β(IL-1β)和肿瘤坏死因子α(TNFα)mRNA表达水平及ELISA法测定IL-6、IL-1β和TNFα蛋白表达水平。结果:HSYA浓度为5×10-6mol/L、10×10-6mol/L和20×10-6mol/L时可抑制LPS(终浓度为1μg/mL)诱导的Eahy926细胞IL-6、IL-1β和TNFαmRNA(模型组vs.正常组P值均<0.01;HSYA干预中高剂量组vs.模型组P值均<0.01;HSYA干预低剂量组vs.模型组,P值均<0.05)和TNFα蛋白(模型组vs.正常组,P值均<0.01;HSYA干预IL-1β表达的中高剂量组vs.模型组,P值<0.05;HSYA干预TNFα表达的中剂量组vs.模型组,P值<0.05;高剂量组vs.模型组,P值<0.01。HSYA干预IL-6表达的低剂量组vs.模型组,P值<0.05;HSYA干预中高剂量组vs.模型组,P值<0.01)表达的升高,并随HSYA剂量升高可见药效增强。结论:HSYA对LPS诱导的Eahy926细胞TLR4、IL-6、IL-1β、TNFα表达升高有抑制作用。Objective：To study the inhibitory effects of HSYA on the increased expression of the um- bilical vein endothelial cells（ Eahy926 cells） inflammatory factor induced by lipopolysaccharide （LPS）. Meth- ods：RT-q PCR method is used to determine the expression levels of toll like receptor 4 （TLR4）, intedeukin 6 （IL-6）, 1 intedeukin （IL-1β） and tumor necrosis factor α（TNF α） mRNA, and ELISA method is used to determine the protein expression levels of IL-6, IL-1β and TNFα. Results： The results showed that after trea- ted with HSYA （5×10-6tool/L, 10×10-6mol/L and 20×10-6mol/L） , the increased expression levels of TLR4, IL-6, IL-1β and TNFα mRNA （ model vs. sham, P 〈 0. O1 ; HSYA 10×10 -6 mol/L and 20×10 -6 mol/L vs. model, P 〈 0.01 ; HSYA 5×10 -6mol/L vs. model, P 〈 0.05 ） and the protein （ model vs. sham, P 〈0.01 ; HSYA vs. model, P 〈0.05 ,or P 〈0. 01） increased expression induced by LPS （1μg/mL ） were inhibited. It was effective. There are significant differences .between the relevant groups. Conclusion： It is sug- gested that the inhibitory effects of HSYA on the increased expression of the endothelial cells（ Eahy926 cells） , TLR4, IL-6, IL-1β and TNF ot is effective.

关 键 词：羟基红花黄色素A 脂多糖 白介素 肿瘤坏死因子Α TOLL样受体4 

分 类 号：R54[医药卫生—心血管疾病]