固相萃取-衍生化-气相色谱法测定化妆品中丙烯酰胺  

Determination of acrylamide in cosmetics using gas chromatography coupled with solid-phase extraction and derivatization

作　　者：李宏亮[1] 詹铭[1] 蒋志华[1] 奚晔 

机构地区：[1]上海市浦东新区疾病预防控制中心,上海200136

出　　处：《中国卫生检验杂志》2012年第7期1499-1501,共3页Chinese Journal of Health Laboratory Technology

基　　金：上海市浦东新区卫生局卫生科技项目(PW2010B-14)

摘　　要：目的:建立化妆品中丙烯酰胺残留的固相萃取-衍生化-气相色谱分析方法。方法:化妆品中丙烯酰胺采用去离子水超声提取,高速离心过滤后固相萃取柱净化,溴化衍生后用乙酸乙酯萃取,经气相色谱电子捕获检测器检测。结果:方法的定量限为0.01 mg/kg,在0.05 mg/kg～0.2 mg/kg 3个添加水平范围内的回收率为78%～94.5%,精密度均小于10.4%。结论:该方法能满足化妆品中丙烯酰胺残留的检测要求。Objective：A comprehensive analytical method based on gas chromatography coupled with solid-phase extraction and derivatization has been developed for the determination of acrylamide residue in cosmetics.Methods： The acrylamide in cosmetics was extracted with water and cleaned up by centrifugal,solid-phase-extraction-column.2,3-DBPA was derived from the extracts through bromination,and extracted by liquid-liquid partition with ethyl acetate.The derivation was determined by GC-ECD.Results： The limit of quantification（LOQ） for acrylamide was 0.01 mg/kg.The mean recoveries were 78%～94.5% at the spiked levels of 0.05 mg/kg～0.2 mg/kg with RSD less than 10.4%.Conclusion： The method is suitable for the determination of acrylamide in cosmetics.

关 键 词：固相萃取 衍生化 气相色谱法 丙烯酰胺 化妆品 

分 类 号：O657.71[理学—分析化学]