人血清精氨酸毛细管电泳紫外检测方法的建立与应用  

Development and application of a capillary electrophoresis method to determine arginine in human serum

作　　者：骆彬[1] 谢颖[1] 贾珊珊[1] 堵亚茹[1] 康维钧[2] 李增宁[1] 

机构地区：[1]河北医科大学第一医院营养科,石家庄050031 [2]河北医科大学公共卫生学院,石家庄050017

出　　处：《中国卫生检验杂志》2012年第7期1517-1519,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立一种准确、快速高效毛细管电泳-紫外检测法测定人血清精氨酸含量的方法。方法:采用高效毛细管电泳法,石英毛细管(70 cm×75μm,有效长度58 cm),分离电压15 kv,检测波长254 nm,高压进样15 s,电泳缓冲溶液比例20 mmol/L pH9.33四硼酸钠:166 mmol/L SDS∶乙腈=10∶10∶3。结果:线性范围为0.03 mmol/L～1.50 mmol/L(r=0.998);以噪声比(S/N)=2时浓度为最低检测限为0.01 mmol/L,精密度为2.4%～4.0%,重复性为2.3%～2.9%,回收率为90.5%～114.2%;检测烧伤患者住院后血中精氨酸含量变化,14 d含量达到高峰。结论:该方法样品预处理简单,分离效果好,可用于实际样品的分析。Objective：To develop a fast and convenient method for determining the content of arginine in human serum.Methods： With high performance capillary electrophoresis,the determination conditions were detection wavelength at 254 nm,fused quartz capillary（70 cm×75 μm,effective length at 58 cm） and a running buffer of 20 mmol/L sodium tetraborate buffer（pH9.33）： 166 mmol/L SDS： acetonitrile=10∶ 10∶ 3 at a constant voltage of 15kV and a sampling time of 15 s.Results： The linearity range was 0.03 mmol/L～1.50 mmol/L（r=0.998）,the detection limit was 0.01 mmol/L,the precision was 2.4%～4.0%,the repeatability was 2.3%～2.9% and the recoveries of sample were 90.5%～114.2%.The arginine level in normal serum was 0.08 mmol/L,while in serum of burn victims reached its peak in 14 days.Conclusion： The method can be used for the determination of arginine in human serum and the assay results were satisfactory.

关 键 词：高效毛细管电泳 紫外检测 血清 精氨酸 烧伤 

分 类 号：O657.32[理学—分析化学]