钴60γ射线灭活人工骨钉PRV病毒效果验证  

Validation of porcine pesudorabies virus inactivation by gamma rays irradiation from cobalt 60

作　　者：钱垂文[1] 张美英[1] 徐斌 王一飞[1] 

机构地区：[1]暨南大学生物医药研发基地/基因工程药物国家工程研究中心,广州510632 [2]广东冠昊生物科技股份有限公司/再生型医用植入器械国家工程实验室,广州510663

出　　处：《中国卫生检验杂志》2012年第7期1573-1575,共3页Chinese Journal of Health Laboratory Technology

基　　金：广东省社会发展领域引导项目(2008B030303064);广州市科技支撑计划(09A71040804)

摘　　要：目的:验证钴60辐照灭活猪源性人工骨钉中指示病毒PRV活性的有效性。方法:首先利用CPE法测定不同辐照剂量γ射线灭活样品后指示病毒的感染活性,初步确定有效辐照剂量;然后利用三批样品对该辐照剂量灭活效果进行验证,辐照前后人工骨钉中指示病毒的滴度采用96孔板CPE法滴定。结果:CPE法测定结果显示,经20kGy及以上辐照剂量处理的样品中指示病毒PRV不能使宿主细胞PK-15出现细胞病变;三批样品经20kGy辐照剂量处理后,指示病毒PRV滴度下降值⊿lgTCID50分别>4.06,>4.75,>4.28,⊿lgTCID50值均大于4。结论:辐照剂量为20kGy的钴60γ射线辐照猪源性人工骨钉可以作为灭活PRV的有效手段。Objective：To validate the indicating virus inactivation in artificial bone screw by Gamma Rays Irradiation from Cobalt 60. Methods： The indicating virus PRV virulence was determined with irradiation at different dosages（10kGy, 15 kGy, 20 kGy, 25 kGy） by cytopathogenic effect（CPE） method, then the effective irradiation dose to inactivate the PRV in artificial bone screw was set, three batch products were used to validate the inactivation effect next. The titer of PRV was assayed with 96-well plate by CPE method. Results： CPE results showed indicating virus PRV in artificial bone screw treated with irradiation dose at 20kGy or more, cannot make PK -15 cells have pathological changes. After irradiation, the logarithm value of PRV titer of three batch product reduced more than 4.06,4.75,4.28 respectively. The logarithm values were more than 4. Conclusion： It was an effective method to inactivate PRV in artificial bone screw with 20kGy irradiation from Cobalt 60.

关 键 词：辐照 人工骨钉 伪狂犬病毒 病毒灭活 

分 类 号：R318.08[医药卫生—生物医学工程]