FPIA法和HPLC法监测丙戊酸血药浓度的比较研究  

Comparison of FPIA and HPLC method in monitoring valproate in epileptic patients plasma

作　　者：王晓秋[1] 温怀凯[1] 朱光辉[1] 余坚[1] 王增寿[1] 邵素倩[1] 

机构地区：[1]温州医学院附属第二医院,浙江温州325027

出　　处：《中国卫生检验杂志》2012年第7期1593-1595,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:比较荧光偏振免疫分析法(FPIA)和高效液相色谱法(HPLC)监测丙戊酸血清药物浓度的相关性。方法:收集癫痫患者服丙戊酸后的稳态谷浓度血样,分别用FPIA法和HPLC法进行测定,考察二种测定方法的相关程度。结果:以HPLC法测定结果(x)与FPIA法测定结果(y)所作线性回归方程如下:y=1.8163+0.8407x(r=0.944),二种方法测定丙戊酸血清药物浓度结果差异有统计学意义(P<0.05)。结论:FPIA法和HPLC法测定丙戊酸血药浓度结果差异具有统计学意义,在丙戊酸治疗药物监测中应予以关注并作相应调整。Objective：To compare Fluorescence polarization immunoassay （FPIA） and high performance liquid chromatography （HPLC） method in monitoring valproate（VPA） in epileptic patients plasma. Methods： Steady-state plasma samples were collected from epileptic patients who had taken valproate. Both FPIA and HPLC method were employed to determine the concentration of VPA. The result were analyzed to obtain the correlation. Then influence of sex and age was evaluated. Results： The regression equation of the results determined by HPLC （X） and FPIA （Y） was y=1.8163＋0.8407x （r=0.944）. The determination results had statistical significance between the two methods（P0.05）. Conclusion： It should pay attention to the difference of 2 kinds of antiepileptic monitoring by FPIA and HPLC method.

关 键 词：丙戊酸 血药浓度测定 荧光偏振免疫分析法 高效液相色谱法 

分 类 号：O657.72[理学—分析化学]