Dissecting the Genetic Basis of Extremely Large Grain Shape in Rice Cultivar 'JZ1560'  

作　　者：Jie-Zheng Ying Ji-Ping Gao Jun-Xiang Shan Mei-Zhen Zhu Min Shi Hong-Xuan Lin 

机构地区：[1]National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research(Shanghai),Shanghai Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,300 Fenglin Road,Shanghai 200032,China [2]National Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China

出　　处：《Journal of Genetics and Genomics》2012年第7期325-333,共9页遗传学报（英文版）

基　　金：supported by grants from the Ministry of Agriculture of China(2009ZX08009-102B);Chinese Academy of Sciences(KSCX3-EW-N-01);the Ministry of Science and Technology of China(2012CB944800);the National Natural Science Foundation of China(31130071);the Zhejiang Provincial Natural Science Foundation of China (Y3110394)

摘　　要：Rice grain shape, grain length （GL）, width （GW）, thickness （GT） and length-to-width ratio （LWR）, are usually controlled by multiple quantitative trait locus （QTL）. To elucidate the genetic basis of extremely large grain shape, QTL analysis was performed using an F2 population derived from a cross between a japonica cuttivar ＇JZI560＇ （extremely large grain） and a contrasting indica cultivar ＇FAZI＇ （small grain）. A total number of 24 QTLs were detected on seven different chromosomes. QTLs for GL, GW, GT and LWR explained 11.6%, 95.62%, 91.5% and 89.9% of total phenotypic variation, respectively. Many QTLs pleiotropically controlled different grain traits, contributing complex traits correlation. GW2 and qSW5/GW5, which have been cloned previously to control GW, showed similar chromosomal locations with qGW2-1/qGT2-1/qLWR2-2 and qGW5-2/qLWR5-1 and should be the right candidate genes. Plants pyramiding GW2 and qSW5/GW5 showed a significant increase in GW compared with those carrying one of the two major QTLs. Furthermore, no significant QTL interaction was observed between GW2 and qSW5/GW5. These results suggested that GW2 and qSW5/GW5 might work in independent pathways to regulate grain traits. ＇JZ1560＇ alleles underlying all QTLs contributed an increase in GW and GT and the accumulation of additive effects generates the extremely large grain shape in ＇JZ1560＇.Rice grain shape, grain length （GL）, width （GW）, thickness （GT） and length-to-width ratio （LWR）, are usually controlled by multiple quantitative trait locus （QTL）. To elucidate the genetic basis of extremely large grain shape, QTL analysis was performed using an F2 population derived from a cross between a japonica cuttivar ＇JZI560＇ （extremely large grain） and a contrasting indica cultivar ＇FAZI＇ （small grain）. A total number of 24 QTLs were detected on seven different chromosomes. QTLs for GL, GW, GT and LWR explained 11.6%, 95.62%, 91.5% and 89.9% of total phenotypic variation, respectively. Many QTLs pleiotropically controlled different grain traits, contributing complex traits correlation. GW2 and qSW5/GW5, which have been cloned previously to control GW, showed similar chromosomal locations with qGW2-1/qGT2-1/qLWR2-2 and qGW5-2/qLWR5-1 and should be the right candidate genes. Plants pyramiding GW2 and qSW5/GW5 showed a significant increase in GW compared with those carrying one of the two major QTLs. Furthermore, no significant QTL interaction was observed between GW2 and qSW5/GW5. These results suggested that GW2 and qSW5/GW5 might work in independent pathways to regulate grain traits. ＇JZ1560＇ alleles underlying all QTLs contributed an increase in GW and GT and the accumulation of additive effects generates the extremely large grain shape in ＇JZ1560＇.

关 键 词：RICE Quantitative trait locus Additive effect Grain shape 

分 类 号：S511-09[农业科学—作物学] S511