豫金银花指标成分的HPLC测定分析  

Measurement and Analysis Benchmark Composition of Lonicera japonica Thunb. Producing in Henan by HPLC

作　　者：李建军[1] 李军芳[1] 梁建强 李景原[1] 周延清[1] 

机构地区：[1]河南师范大学生命科学学院,河南省高校道地中药材保育及利用工程技术研究中心,河南新乡453007 [2]新乡佐今明制药股份有限公司,河南新乡453000

出　　处：《广西师范大学学报（自然科学版）》2012年第2期94-98,共5页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家"十一五"科技支撑计划项目(2006BRI06R12-06);河南省重点科技攻关项目(112102310019);河南省教育厅自然科学研究计划项目(2011A180017)

摘　　要：采用高效液相色谱法测定豫金银花指标成分,分析密银花与封银花的差异及封银花不同部位的成分差异。结果表明,绿原酸含量:封丘红银花3.10%(对照)>封丘大毛花2.87%(p<0.05)>密县大毛花2.22%(p<0.01),木犀草苷含量:封丘红银花0.181%(对照)>封丘大毛花0.095%(p<0.05)>密县大毛花0.075%(p<0.05);封丘大毛花不同部位绿原酸含量:花蕾2.87%(对照)>叶0.84%(p<0.01)>芽尖0.67%(p<0.01)>枝0.61%(p<0.01),木犀草苷含量:芽尖0.488%(对照)>叶0.397%(p<0.01)>花蕾0.095%(p<0.01)>枝0.066%(p<0.01);封丘红银花不同部位绿原酸含量:花蕾3.10%(对照)>叶1.34%(p<0.01)>芽尖1.29%(p<0.01)>枝0.92%(p<0.01),木犀草苷含量:芽尖0.329%(对照)>叶0.299%(p<0.01)>花蕾0.181%(p<0.01)>枝0.063%(p<0.01)。豫金银花花蕾的指标性成分含量均高于药典规定的最低标准且质量较好,相较而言封银花的品质优于密银花,叶和芽尖可为金银花综合开发提供新原料。The differences between Mixian honeysuckle and Fengqiu honeysuckle and the differences among different parts of Fengqiu honeysuckle were analyzed by high performance liquid chromatogra- phy. The results indicated that chlorogenic acid content： Fengqiu red honeysuckle 3. 10%（contrast） Fengqiu honeysuckle 0. 095% （p〈0. 05））Mixian honeysuckle 2. 22% （p〈0. 01）;luteoloside content ： Fengqiu red honeysuckle 0. 181% （contrast） 〉Fengqiu honeysuckle 0. 095% （p（0.05） ~Mixian honey- suckle 0. 075 % （p〈0.05） ;ehlorogenic acid content in different parts of Fengqiu honeysuckle ：flower bud 2. 87% （contrast）〉leaf 0. 84% （p（0. 01）〉bud 0. 67% （p（0.01）〉branch 0.61% （p〈0.01）,Luteoloside content：bud 0. 488%（contrast）〉leaf 0. 397%（p〈0.01）〉flower bud 0. 095%（p〈0. 01））branch 0. 066% （p〈0, 01） ;chlorogenie acid content in different parts of Fengqiu tad honeysuckle：flower bud 3.10% （contrast））leaf 1.34%（p〈0. 01）〉bud 1.29% （p（0.01））branch 0. 92% （p（0. 01）,Luteolo-side content.bud 0. 329%（contrast）〉leaf 0. 299% （p（0.01）〉flower bud 0, 181%（p〈0.01））branch 0. 063 % （p〈0.01）. The contents of components of honeysuckle Yu were higher than the minimun standards of China Pharmacopeia （2010ed） and the quality of Fengqiu honeysuckle superior quality of Mixian honeysuckle ;Leaf and bud can provide new materials for the comprehensive development of honeysuckle.

关 键 词：豫金银花 高效液相色谱 绿原酸 木犀草苷 

分 类 号：R931.6[医药卫生—生药学]