慢性乙型肝炎肝郁脾虚证和脾胃湿热证患者的差异表达基因研究初探  

Study on the Differential Gene Expressions of Chronic Hepatitis B Patients of Gan Depression Pi Deficiency Syndrome and Pi-Wei Damp-heat Syndrome

作　　者：杨婵娟[1] 刘宏伟 王丽春[1] 张传涛[3] 冯全生[3] 范昕建[1,3] 

机构地区：[1]四川大学华西医院感染性疾病中心,成都610041 [2]生物芯片上海国家工程中心,上海201203 [3]成都中医药大学,成都610075

出　　处：《中国中西医结合杂志》2012年第8期1032-1037,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家科技重大专项资助项目--病毒性肝炎中医证候生物学技术平台研究(No.2009ZX10005-020)

摘　　要：目的探讨慢性乙型肝炎(慢乙肝)肝郁脾虚证与脾胃湿热证患者的差异表达基因。方法采集正常健康者(26名)及肝郁脾虚证(35例)、脾胃湿热证(34例)患者空腹肘静脉血,按Trizol法提取总RNA。采用Aglient表达谱芯片进行基因芯片检测,利用随机方差模型筛选差异基因,通过GO、Pathway、Gen-bank、NCBI、Geneontology等数据库对差异基因进行分析。结果两证型间获得差异基因125个(其中66个为上调基因,59个为下调基因),主要表现的功能为跨膜运输、硒反应离子、钙离子依赖的胞吐作用等。参与的通路中较为重要的包括影响细胞黏附分子、钙离子信号通路、白细胞穿上皮迁移等。通过动态网络构建,寻找出共表达能力差异最显著的9个基因(即LOC340508、HIST2H2BE、MPL、FLJ22536、TUBA8、NT5M、EG-FL7、PTPRF、TSPAN33),其主要涉及免疫反应、细胞生长、DNA损伤、信号转导、炎症反应等生命过程。结论慢乙肝肝郁脾虚证和脾胃湿热证两个证型间存在差异表达基因,提示中医证候分类学具有基因表达谱依据,基因组学研究方法有望为中医辨证提供客观依据。Objective To explore the differential gene expressions of chronic hepatitis B （CHB） of Gan depression Pi deficiency syndrome （GDPDS） and Pi-Wei damp-heat syndrome （PWDHS）. Methods The ulnar venous blood was withdrawn from healthy subjects （26 cases）, patients of GDPDS （35 cases） and PWDHS （34 cases） on an empty stomach. The total RNA was extracted using Trizol method. The differential genes were de- tected using Aglient expression profile chip and screened using randomized variance model. The results were an- alyzed using GO, Pathway, GeneBank, NCBI, and Geneontology. Results There were 125 differential genes between CHB patients of GDPDS and those of PWDHS （ including 66 up-regulated genes and 59 down-regulated genes）, mainly involving in functions of transmembrane transport, response to selenium ion, and regulation of calcium ion-dependent exocytosis. The signal pathway participated in mainly includes cell adhesion molecules, calcium ion signaling pathway, leukocyte trans-endothelial migration. We present gene co-expression networks to find 9 interactions among genes （LOC340508, HIST2H2BE, MPL, FLJ22536, TUBA8, NT5M, EGFLT, PT- PRF, TSPAN33）, which were mainly involved in immune response, cell growth, DNA damage, signal transduc- tion, inflammatory reaction, and so on. Conclusions The differential expression genes existed between CHB pa- tients of GDPDS and those of PWDHS, indicating that Chinese medicine syndrome classification has its own ba- sis for gene expression profile. The genomics research method is expected to provide an objective basis for Chi- nese medicine syndrome typing.

关 键 词：慢性乙型肝炎 肝郁脾虚证 脾胃湿热证 差异表达基因 基因芯片 

分 类 号：R259[医药卫生—中西医结合]